Recombinant
RabMAb

Recombinant Anti-Bcl2 alpha antibody [SP66] - N-terminal (ab93884)

Overview

  • Product name

    Anti-Bcl2 alpha antibody [SP66] - N-terminal
    See all Bcl2 alpha primary antibodies
  • Description

    Rabbit monoclonal [SP66] to Bcl2 alpha - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Bcl2 alpha aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: P10415

  • Positive control

    • IHC-P: Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab93884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.
  • Cellular localization

    Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
  • Database links

  • Alternative names

    • Apoptosis regulator Bcl2 antibody
    • B cell lymphoma protein 2 alpha isoform antibody
    • B cell lymphoma protein 2 antibody
    • B-cell CLL/lymphoma 2 antibody
    • B-cell leukemia/lymphoma 2 antibody
    • Bcl 2 antibody
    • Leukemia /lymphoma, B-cell, 2 antibody
    • Oncogene B-cell leukemia 2 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Bcl2 alpha with ab93884 at 1/100 dilution in immunohistochemical analysis. DAB staining.

References

ab93884 has not yet been referenced specifically in any publications.

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