Recombinant
RabMAb

Recombinant Anti-Bcl2-L-13 antibody [EP10625] (ab203516)

Overview

  • Product name

    Anti-Bcl2-L-13 antibody [EP10625]
    See all Bcl2-L-13 primary antibodies
  • Description

    Rabbit monoclonal [EP10625] to Bcl2-L-13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human Bcl2-L-13 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BXK5

  • Positive control

    • WB: Raji, K562, THP-1 and HeLa cell lysates. IHC-P: Human pancreas and tonsil tissues; Mouse cardiac muscle tissue; Rat kidney tissue.
  • General notes

     

     

    Previously labelled as Bcl rambo.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP10625
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 85 kDa (predicted molecular weight: 53 kDa).

Target

  • Function

    May promote the activation of caspase-3 and apoptosis.
  • Tissue specificity

    Ubiquitous, with the highest levels of expression in heart, placenta and pancreas.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Cellular localization

    Nucleus and Mitochondrion membrane. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis facilitator antibody
    • B2L13_HUMAN antibody
    • Bcl 2 like 13 antibody
    • Bcl 2 like 13 protein antibody
    • Bcl rambo antibody
    • Bcl-2-like protein 13 antibody
    • Bcl-rambo antibody
    • BCL2 like 13 apoptosis facilitator antibody
    • BCL2 like 13 antibody
    • Bcl2 like 13 protein antibody
    • Bcl2-L-13 antibody
    • BCL2L13 antibody
    • MIL 1 antibody
    • MIL1 antibody
    • MIL1 protein antibody
    • Protein Mil1 antibody
    see all

Images

  • Anti-Bcl2-L-13 antibody [EP10625] (ab203516) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • All lanes : Anti-Bcl2-L-13 antibody [EP10625] (ab203516) at 1/5000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 2 : THP-1 (Human monocytic leukemia cell line) cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic and nuclear staining on human pancreas tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic and nuclear staining on human tonsil tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic and nuclear staining on mouse cardiac muscle tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic and nuclear staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

References

ab203516 has not yet been referenced specifically in any publications.

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