Key features and details
- Rabbit polyclonal to BCL2L10
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BCL2L10 antibody
See all BCL2L10 primary antibodies
DescriptionRabbit polyclonal to BCL2L10
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 1-194 of Human BCL2L10 (UniProt ID: Q9HD36).
- A431, H1299, HeLa and HepG2 whole cell lysates; AGS xenograft; HeLa cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 22 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionPromotes cell survival. Suppresses apoptosis induced by BAX but not BAK.
Tissue specificityWidely expressed in adult tissues. Preferentially expressed in lung, liver and kidney.
Sequence similaritiesBelongs to the Bcl-2 family.
Cellular localizationMitochondrion. Nucleus membrane.
- Information by UniProt
- Anti apoptotic protein NrH antibody
- Anti-apoptotic protein NrH antibody
- Apoptosis regulator Bcl B antibody
All lanes : Anti-BCL2L10 antibody (ab151419) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12% SDS PAGE
Immunohistochemical analysis of paraffin-embedded AGS xenograft labeling BCL2L10 with ab151419 at 1/500 dilution.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling BCL2L10 with ab151419 at 1/200 dilution.
The image in the lower panel shows cells co-stained with Hoechst 33342.
ab151419 has not yet been referenced specifically in any publications.