Product nameAnti-Bcl2L2 antibody
DescriptionRabbit polyclonal to Bcl2L2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 20 kDa.
The detection limit for Bcl2L2 is approximately 0.5 ng/lane under reducing conditions.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPromotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
Tissue specificityExpressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines.
Sequence similaritiesBelongs to the Bcl-2 family.
DomainThe BH4 motif seems to be involved in the anti-apoptotic function.
The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. The C-terminal residues of BCL2L2 fold into the BH3-binding cleft and modulate pro-survival activity by regulating ligand access. When BH3 domain-containing proteins bind, they displace the C-terminus, allowing its insertion into the membrane and neutralizing the pro-survival activity of BCL2L2.
Cellular localizationMitochondrion membrane. Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane.
- Information by UniProt
- Apoptosis regulator BCL W antibody
- Apoptosis regulator Bcl-W antibody
- B2CL2_HUMAN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Bcl2L2 using ab190952 at 1 μg/ml in human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
All lanes : Anti-Bcl2L2 antibody (ab190952) at 0.5 µg/ml
Lane 1 : human A549 whole cell lysates
Lane 2 : human K562 whole cell lysates
Lane 3 : human U-937 whole cell lysates
Lane 4 : human HeLa whole cell lysates
Lane 5 : human A431 whole cell lysates
Lysates/proteins at 50 µg per lane.
All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 20 kDa
The membrane was blocked with 5% Non-fat Milk/ TBS for 1.5 hour at RT.
ab190952 has not yet been referenced specifically in any publications.