Overview

  • Product name

    Anti-Bcl2L2 antibody
  • Description

    Rabbit polyclonal to Bcl2L2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Bcl2L2 aa 74-88 (internal sequence).
    Sequence:

    SAQQRFTQVSDELFQ


    Database link: Q92843

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 20 kDa.

The detection limit for Bcl2L2 is approximately 0.5 ng/lane under reducing conditions.

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
  • Tissue specificity

    Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    The BH4 motif seems to be involved in the anti-apoptotic function.
    The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. The C-terminal residues of BCL2L2 fold into the BH3-binding cleft and modulate pro-survival activity by regulating ligand access. When BH3 domain-containing proteins bind, they displace the C-terminus, allowing its insertion into the membrane and neutralizing the pro-survival activity of BCL2L2.
  • Cellular localization

    Mitochondrion membrane. Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis regulator BCL W antibody
    • Apoptosis regulator Bcl-W antibody
    • B2CL2_HUMAN antibody
    • BCL 2 Like 2 antibody
    • Bcl 2 like 2 protein antibody
    • Bcl 2L2 antibody
    • BCL W antibody
    • Bcl-2-like protein 2 antibody
    • Bcl2 L2 antibody
    • BCL2 like 2 antibody
    • BCL2 like 2 protein antibody
    • Bcl2-L-2 antibody
    • Bcl2l2 antibody
    • BCLW antibody
    • KIAA0271 antibody
    • PPP1R51 antibody
    • Protein phosphatase 1 regulatory subunit 51 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Bcl2L2 using ab190952 at 1 μg/ml in human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
  • All lanes : Anti-Bcl2L2 antibody (ab190952) at 0.5 µg/ml

    Lane 1 : human A549 whole cell lysates
    Lane 2 : human K562 whole cell lysates
    Lane 3 : human U-937 whole cell lysates
    Lane 4 : human HeLa whole cell lysates
    Lane 5 : human A431 whole cell lysates

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution

    Predicted band size: 20 kDa



    The membrane was blocked with 5% Non-fat Milk/ TBS for 1.5 hour at RT.

References

ab190952 has not yet been referenced specifically in any publications.

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