Overview

  • Product name
  • Description
    Rabbit polyclonal to Bcl2L2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    This antibody was developed against a synthetic peptide corresponding within amino acid residues 1-100 of human Bcl2L2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 21289171
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 21 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
  • Tissue specificity
    Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    The BH4 motif seems to be involved in the anti-apoptotic function.
    The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. The C-terminal residues of BCL2L2 fold into the BH3-binding cleft and modulate pro-survival activity by regulating ligand access. When BH3 domain-containing proteins bind, they displace the C-terminus, allowing its insertion into the membrane and neutralizing the pro-survival activity of BCL2L2.
  • Cellular localization
    Mitochondrion membrane. Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator BCL W antibody
    • Apoptosis regulator Bcl-W antibody
    • B2CL2_HUMAN antibody
    • BCL 2 Like 2 antibody
    • Bcl 2 like 2 protein antibody
    • Bcl 2L2 antibody
    • BCL W antibody
    • Bcl-2-like protein 2 antibody
    • Bcl2 L2 antibody
    • BCL2 like 2 antibody
    • BCL2 like 2 protein antibody
    • Bcl2-L-2 antibody
    • Bcl2l2 antibody
    • BCLW antibody
    • KIAA0271 antibody
    • PPP1R51 antibody
    • Protein phosphatase 1 regulatory subunit 51 antibody
    see all

Images

  • Anti-Bcl2L2 antibody (ab38629) + HL-60 cell lysate

    Predicted band size: 21 kDa
    Observed band size: 25 kDa (why is the actual band size different from the predicted?)



    Secondary HRP-anti-rabbit was used for signal visualisation with chemiluminescence.
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References

This product has been referenced in:
  • Tian L  et al. Calotropin regulates the apoptosis of non-small cell cancer by regulating the cytotoxic T-lymphocyte associated antigen 4-mediated TGF-ß/ERK signaling pathway. Mol Med Rep 17:7683-7691 (2018). Read more (PubMed: 29620207) »
  • Kamalidehghan B  et al. Inhibition of human prostate cancer (PC-3) cells and targeting of PC-3-derived prostate cancer stem cells with koenimbin, a natural dietary compound from Murraya koenigii (L) Spreng. Drug Des Devel Ther 12:1119-1133 (2018). Read more (PubMed: 29765202) »

See all 6 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS cells)
Permeabilization
Yes - Tween-20
Specification
U2OS cells
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Dec 28 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Liver Tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
Permeabilization
Yes - Tween-20
Specification
Human Liver Tissue
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 28 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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