Anti-Bcl3 antibody (ab216877)
Key features and details
- Rabbit polyclonal to Bcl3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl3 antibody
See all Bcl3 primary antibodies -
Description
Rabbit polyclonal to Bcl3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Bcl3 overexpression HEK-293T whole cell lysate; recombinant Bcl3 protein; U-87 MG, C2C12 and NIH/3T3 whole cell lysates. ICC/IF: U-87 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation. -
Involvement in disease
Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions. -
Sequence similarities
Contains 7 ANK repeats. -
Post-translational
modificationsPolyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. - Information by UniProt
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Database links
- Entrez Gene: 602 Human
- Entrez Gene: 12051 Mouse
- Omim: 109560 Human
- SwissProt: P20749 Human
- SwissProt: Q9Z2F6 Mouse
- Unigene: 31210 Human
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Alternative names
- B cell CLL/lymphoma 3 antibody
- B cell leukemia/lymphoma 3 antibody
- B cell lymphoma 3 encoded protein antibody
see all
Images
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U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for Bcl3 (green) using ab216877 at 10 µg/ml overnight at 4ºC in ICC/IF. The secondary antibody is Anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed for at 5 μg/ml for 1 hour at room temperature. Cells were fixed in 4% paraformaldehyde.
The nuclear counterstain is DAPI (blue).
A: DAPI
B: Primary followed by Anti-rabbit IgG Dylight™ 488
C: Merged images
D: Secondary only control
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All lanes : Anti-Bcl3 antibody (ab216877) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Bcl3 overexpression HEK-293T, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : DyLight™ 488 Conjugated Pre-Adsorbed antibody at 1/20000 dilution
Predicted band size: 48 kDaDilution buffer and concentration: PBS/0.01% sodium azide
Observed MW ~48 kDa ubiquitination, phosphorylation
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All lanes : Anti-Bcl3 antibody (ab216877) at 1 µg/ml
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit secondary HRP antibody at 1/70000 dilution
Predicted band size: 48 kDaBlocking buffer and concentration: 1% Casein
Dilution buffer and concentration: PBS/0.01% sodium azide
Observed MW ~47 kDa endogenous protein
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Anti-Bcl3 antibody (ab216877) at 1/1000 dilution + Recombinant human Bcl3 at 0.5 µg
Secondary
DyLight™ 488 Conjugated Pre-Adsorbed antibody at 1/20000 dilution
Predicted band size: 48 kDaDilution buffer and concentration: PBS/0.01% sodium azide
Observed MW ~75 kDa on recombinant protein.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab216877 has been referenced in 2 publications.
- Wang J et al. Repression of the miR-627-5p by histone deacetylase 3 contributes to hypoxia-induced hepatocellular carcinoma progression. J Cancer 12:5320-5330 (2021). PubMed: 34335948
- Li BB et al. Cell invasion in digital microfluidic microgel systems. Sci Adv 6:eaba9589 (2020). PubMed: 32832633