Overview

  • Product name

  • Description

    Rabbit polyclonal to Bcl3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control

    • WB: Bcl3 overexpression HEK-293T whole cell lysate; recombinant Bcl3 protein; U-87 MG, C2C12 and NIH/3T3 whole cell lysates. ICC/IF: U-87 MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab216877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.
  • Involvement in disease

    Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.
  • Sequence similarities

    Contains 7 ANK repeats.
  • Post-translational
    modifications

    Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
    Activated by phosphorylation.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell CLL/lymphoma 3 antibody
    • B cell leukemia/lymphoma 3 antibody
    • B cell lymphoma 3 encoded protein antibody
    • B-cell lymphoma 3 protein antibody
    • Bcl 3 antibody
    • BCL 4 antibody
    • BCL-3 antibody
    • Bcl3 antibody
    • BCL3_HUMAN antibody
    • BCL4 antibody
    • Chronic lymphatic leukemia protein antibody
    • D19S37 antibody
    • Protein Bcl 3 antibody
    • Proto-oncogene BCL3 antibody
    see all

Images

  • U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for Bcl3 (green) using ab216877 at 10 µg/ml overnight at 4ºC in ICC/IF. The secondary antibody is Anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed for at 5 μg/ml for 1 hour at room temperature. Cells were fixed in 4% paraformaldehyde.

    The nuclear counterstain is DAPI (blue).

    A: DAPI

    B: Primary followed by Anti-rabbit IgG Dylight™ 488

    C: Merged images

    D: Secondary only control

  • All lanes : Anti-Bcl3 antibody (ab216877) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Bcl3 overexpression HEK-293T, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : DyLight™ 488 Conjugated Pre-Adsorbed antibody at 1/20000 dilution

    Predicted band size: 48 kDa



    Dilution buffer and concentration: PBS/0.01% sodium azide

    Observed MW ~48 kDa ubiquitination, phosphorylation

  • All lanes : Anti-Bcl3 antibody (ab216877) at 1 µg/ml

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : C2C12 (mouse myoblast cell line) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit secondary HRP antibody at 1/70000 dilution

    Predicted band size: 48 kDa



    Blocking buffer and concentration: 1% Casein

    Dilution buffer and concentration: PBS/0.01% sodium azide

    Observed MW ~47 kDa endogenous protein

  • Anti-Bcl3 antibody (ab216877) at 1/1000 dilution + Recombinant human Bcl3 at 0.5 µg

    Secondary
    DyLight™ 488 Conjugated Pre-Adsorbed antibody at 1/20000 dilution

    Predicted band size: 48 kDa



    Dilution buffer and concentration: PBS/0.01% sodium azide

    Observed MW ~75 kDa on recombinant protein.

References

ab216877 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab216877.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up