Key features and details
- Rabbit polyclonal to Bcl3
- Suitable for: WB, IP, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Bcl3 antibody
See all Bcl3 primary antibodies
DescriptionRabbit polyclonal to Bcl3
Tested applicationsSuitable for: WB, IP, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (Human) - mapping to the C terminus of Bcl 3.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab27780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).|
|IP||Use a concentration of 5 - 10 µg/ml.|
|IHC-Fr||Use a concentration of 20 - 40 µg/ml.|
FunctionContributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.
Involvement in diseaseNote=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.
Sequence similaritiesContains 7 ANK repeats.
modificationsPolyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.
- Information by UniProt
- B cell CLL/lymphoma 3 antibody
- B cell leukemia/lymphoma 3 antibody
- B cell lymphoma 3 encoded protein antibody
ab27780 has been referenced in 2 publications.
- Shao M et al. MYBL2 protects against H9c2 injury induced by hypoxia via AKT and NF-?B pathways. Mol Med Rep 17:4832-4838 (2018). PubMed: 29328450
- Long TY et al. CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-?B pathway. Braz J Med Biol Res 50:e5861 (2017). PubMed: 28355355