Recombinant Anti-Bcl3 antibody [EPR23835-115] (ab259832)
![Western blot - Anti-Bcl3 antibody [EPR23835-115] (ab259832)](https://www.abcam.com/ps/products/259/ab259832/Images/ab259832-450348-anti-bcl3-antibody-epr23835115-western-blot-carcinoma.jpg "Western blot - Anti-Bcl3 antibody [EPR23835-115] (ab259832)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23835-115] to Bcl3
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Bcl3 antibody [EPR23835-115]
See all Bcl3 primary antibodies -
Description
Rabbit monoclonal [EPR23835-115] to Bcl3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Bcl3 aa 100-400. The exact sequence is proprietary.
Database link: P20749 -
Positive control
- WB: A549, C2C12 and HeLa whole cell lysate. Mouse ovary tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23835-115 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab259832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 48 kDa. |
Target
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Function
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation. -
Involvement in disease
Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions. -
Sequence similarities
Contains 7 ANK repeats. -
Post-translational
modificationsPolyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. - Information by UniProt
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Database links
- Entrez Gene: 602 Human
- Entrez Gene: 12051 Mouse
- Omim: 109560 Human
- SwissProt: P20749 Human
- SwissProt: Q9Z2F6 Mouse
- Unigene: 31210 Human
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Alternative names
- B cell CLL/lymphoma 3 antibody
- B cell leukemia/lymphoma 3 antibody
- B cell lymphoma 3 encoded protein antibody
see all
Images
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Western blot - Anti-Bcl3 antibody [EPR23835-115] (ab259832)All lanes : Anti-Bcl3 antibody [EPR23835-115] (ab259832) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 2 : mouse ovary tissue lysate
Lane 3 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Fresh lysate was used in lane 1.
Exposure time: Lane 1: 5.5 seconds; Lane 2-3: 3 minutes.
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![Western blot - Anti-Bcl3 antibody [EPR23835-115] (ab259832)](https://www.abcam.com/ps/products/259/ab259832/Images/ab259832-450349-anti-bcl3-antibody-epr23835115-western-blot-adenocarcinoma.jpg)
Western blot - Anti-Bcl3 antibody [EPR23835-115] (ab259832)All lanes : Anti-Bcl3 antibody [EPR23835-115] (ab259832) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : THP-1 (human monocytic leukemia monocyte), whole cell lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: THP-1 (PMID: 25089799).
Exposure time: 81 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab259832 has not yet been referenced specifically in any publications.
