Key features and details
- Mouse monoclonal [BCL6/1526] to Bcl6 - BSA and Azide free
- Suitable for: IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Bcl6 antibody [BCL6/1526] - BSA and Azide free
See all Bcl6 primary antibodies
DescriptionMouse monoclonal [BCL6/1526] to Bcl6 - BSA and Azide free
Tested applicationsSuitable for: IHC-P, Protein Arraymore details
Species reactivityReacts with: Human
Recombinant fragment within Human Bcl6 aa 256-389. The exact sequence is proprietary.
Database link: P41182
- Human tonsil tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein G purified
Purification notesab218951 was purified from Bioreactor Concentrate by Protein A/G.
Our Abpromise guarantee covers the use of ab218951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 30 min at RT).
|Protein Array||Use at an assay dependent concentration.|
FunctionTranscriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis.
Tissue specificityExpressed in germinal center T and B cells and in primary immature dendritic cells.
Involvement in diseaseNote=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF.
Sequence similaritiesContains 1 BTB (POZ) domain.
Contains 6 C2H2-type zinc fingers.
DomainThe BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2.
modificationsPhosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway.
- Information by UniProt
- B cell CLL/lymphoma 6 antibody
- B cell lymphoma 6 protein antibody
- B-cell lymphoma 5 protein antibody
This data was produced with ab218508, the same antibody in a different formulation with BSA and Azide.
ab218508 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Bcl6 with ab218951 at 2 µg/ml.
ab218951 has not yet been referenced specifically in any publications.