Overview

  • Product name
    Anti-Bcl6 antibody [EP529Y]
    See all Bcl6 primary antibodies
  • Description
    Rabbit monoclonal [EP529Y] to Bcl6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Bcl6 aa 650-750 (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Daudi and Ramos cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab33901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 85-90 kDa (predicted molecular weight: 78 kDa).

Target

  • Function
    Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis.
  • Tissue specificity
    Expressed in germinal center T and B cells and in primary immature dendritic cells.
  • Involvement in disease
    Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
    Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
    Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF.
  • Sequence similarities
    Contains 1 BTB (POZ) domain.
    Contains 6 C2H2-type zinc fingers.
  • Domain
    The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2.
  • Post-translational
    modifications
    Phosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell CLL/lymphoma 6 antibody
    • B cell lymphoma 6 protein antibody
    • B-cell lymphoma 5 protein antibody
    • B-cell lymphoma 6 protein antibody
    • BCL 5 antibody
    • Bcl 6 antibody
    • BCL-5 antibody
    • BCL-6 antibody
    • BCL5 antibody
    • BCL6 antibody
    • BCL6_HUMAN antibody
    • BCL6A antibody
    • cys his2 zinc finger transcription factor antibody
    • LAZ 3 antibody
    • LAZ 3 protein antibody
    • LAZ3 antibody
    • Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) antibody
    • Lymphoma associated zinc finger gene on chromosome 3 antibody
    • Protein LAZ-3 antibody
    • ZBTB 27 antibody
    • ZBTB27 antibody
    • Zinc finger and BTB domain containing protein 27 antibody
    • Zinc finger and BTB domain-containing protein 27 (ZBTB27) antibody
    • Zinc finger and BTB domain-containing protein 27 antibody
    • Zinc Finger Protein 51 (ZNF51) antibody
    • Zinc finger protein 51 antibody
    • zinc finger transcription factor BCL6S antibody
    • ZNF 51 antibody
    • ZNF51 antibody
    see all

Images

  • All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution (Purified)

    Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
    Lane 2 : Ramos (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa


    Exposure time: 10 seconds


    Blocking and diluting buffer: 5% NFDM/TBST.

    This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution (Purified)

    Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in RIPA lysis method
    Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST.

    This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).

  • Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/2000 dilution (Unpurified) + Daudi cell lysate

    Predicted band size: 78 kDa
    Observed band size: 85-90 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Kuai Y  et al. Wilms' tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90. Cell Commun Signal 16:50 (2018). Read more (PubMed: 30143009) »
  • Sato F  et al. Angioimmunoblastic T-cell lymphoma mice model. Leuk Res 37:21-7 (2013). Read more (PubMed: 23026775) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (canine B cell lymphoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
canine B cell lymphoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 16 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (DLBCL cell line SUDHL6 xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
DLBCL cell line SUDHL6 xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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