Recombinant Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Bcl6 antibody [EP529Y] - BSA and Azide free
See all Bcl6 primary antibodies -
Description
Rabbit monoclonal [EP529Y] to Bcl6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Bcl6 aa 650-750 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Daudi and Ramos cell lysates.
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General notes
ab240925 is the carrier-free version of ab33901 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240925 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP529Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab240925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 85-90 kDa (predicted molecular weight: 78 kDa). |
Target
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Function
Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis. -
Tissue specificity
Expressed in germinal center T and B cells and in primary immature dendritic cells. -
Involvement in disease
Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 6 C2H2-type zinc fingers. -
Domain
The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2. -
Post-translational
modificationsPhosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 604 Human
- Omim: 109565 Human
- SwissProt: P41182 Human
- Unigene: 478588 Human
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Alternative names
- B cell CLL/lymphoma 6 antibody
- B cell lymphoma 6 protein antibody
- B-cell lymphoma 5 protein antibody
see all
Images
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![Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)](https://www.abcam.com/ps/products/240/ab240925/Images/ab240925-330681-anti-bcl6-antibody-ep529y-western-blot.jpg)
Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
Lane 2 : Ramos (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Exposure time: 10 secondsBlocking and diluting buffer: 5% NFDM/TBST.
This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab33901).
Datasheets and documents
References
ab240925 has not yet been referenced specifically in any publications.
