Recombinant Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)
![Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)](https://www.abcam.com/ps/products/240/ab240925/Images/ab240925-384886-anti-bcl6-antibody-ep529y-bsa-and-azide-free-western-blot-hela-ramos-sh-sy5y.jpg "Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP529Y] to Bcl6 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Bcl6 antibody [EP529Y] - BSA and Azide free
See all Bcl6 primary antibodies -
Description
Rabbit monoclonal [EP529Y] to Bcl6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Bcl6 aa 650-750 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Daudi and Ramos, HeLa and SH-SY5Y cell lysates.
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General notes
ab240925 is the carrier-free version of ab33901 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240925 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP529Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab240925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 85-90 kDa (predicted molecular weight: 78 kDa). |
Target
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Function
Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis. -
Tissue specificity
Expressed in germinal center T and B cells and in primary immature dendritic cells. -
Involvement in disease
Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 6 C2H2-type zinc fingers. -
Domain
The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2. -
Post-translational
modificationsPhosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 604 Human
- Omim: 109565 Human
- SwissProt: P41182 Human
- Unigene: 478588 Human
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Alternative names
- B cell CLL/lymphoma 6 antibody
- B cell lymphoma 6 protein antibody
- B-cell lymphoma 5 protein antibody
see all
Images
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Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BCL6 knockout HeLa cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab33901).
Lanes 1-4: Merged signal (red and green). Green ab33901 observed at 78 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab33901 Anti-Bcl6 antibody [EP529Y] was shown to react with BCL6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265410 (knockout cell lysate ab257178) was used. Wild-type and Bcl6 knockout samples were subjected to SDS-PAGE. ab33901 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)](https://www.abcam.com/ps/products/240/ab240925/Images/ab240925-330681-anti-bcl6-antibody-ep529y-western-blot.jpg)
Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
Lane 2 : Ramos (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Exposure time: 10 secondsBlocking and diluting buffer: 5% NFDM/TBST.
This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab33901).
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![Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)](https://www.abcam.com/ps/products/240/ab240925/Images/ab240925-3-benefits-of-recombinant-antibodies.png)
Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (ab240925)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab240925 has not yet been referenced specifically in any publications.
