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  1. Link

    bcl6-antibody-epr11410-43-ab172610.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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RecombinantRabMAb

Recombinant Anti-Bcl6 antibody [EPR11410-43] (ab172610)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (8)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
  • Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
  • Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
  • Anti-Bcl6 antibody [EPR11410-43] (ab172610)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11410-43] to Bcl6
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Bcl6 antibody [EPR11410-43]
    See all Bcl6 primary antibodies
  • Description

    Rabbit monoclonal [EPR11410-43] to Bcl6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human bcl-6 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P41182

  • Positive control

    • Ramos, Daudi and Raji cell lysates; Human lymph node, tonsil and B cell lymphoma tissues; Ramos cells.
  • General notes

    We tested the antibody in mouse and rat samples and tests were unsuccessful therefore we can no longer guaranteed the reactivity of this species.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11410-43
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Alternative Versions

    • Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Daudi whole cell lysate (ab3951)
    • Ramos whole cell lysate (ab3955)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 87-98 kDa (predicted molecular weight: 78 kDa).
IHC-P (3)
1/1500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/200 - 1/500. See IHC antigen retrieval protocols.

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 87-98 kDa (predicted molecular weight: 78 kDa).
IHC-P
1/1500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/200 - 1/500. See IHC antigen retrieval protocols.

Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis.
  • Tissue specificity

    Expressed in germinal center T and B cells and in primary immature dendritic cells.
  • Involvement in disease

    Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
    Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
    Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF.
  • Sequence similarities

    Contains 1 BTB (POZ) domain.
    Contains 6 C2H2-type zinc fingers.
  • Domain

    The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2.
  • Post-translational
    modifications

    Phosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P41182 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 604 Human
    • Omim: 109565 Human
    • SwissProt: P41182 Human
    • Unigene: 478588 Human
    • Alternative names

      • B cell CLL/lymphoma 6 antibody
      • B cell lymphoma 6 protein antibody
      • B-cell lymphoma 5 protein antibody
      • B-cell lymphoma 6 protein antibody
      • BCL 5 antibody
      • Bcl 6 antibody
      • BCL-5 antibody
      • BCL-6 antibody
      • BCL5 antibody
      • BCL6 antibody
      • BCL6_HUMAN antibody
      • BCL6A antibody
      • cys his2 zinc finger transcription factor antibody
      • cys-his2 zinc finger transcription factor antibody
      • LAZ 3 antibody
      • LAZ 3 protein antibody
      • LAZ3 antibody
      • Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) antibody
      • Lymphoma associated zinc finger gene on chromosome 3 antibody
      • Protein LAZ-3 antibody
      • ZBTB 27 antibody
      • ZBTB27 antibody
      • Zinc finger and BTB domain containing protein 27 antibody
      • Zinc finger and BTB domain-containing protein 27 (ZBTB27) antibody
      • Zinc finger and BTB domain-containing protein 27 antibody
      • Zinc Finger Protein 51 (ZNF51) antibody
      • Zinc finger protein 51 antibody
      • zinc finger transcription factor BCL6S antibody
      • ZNF 51 antibody
      • ZNF51 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node in stomach tissue sections labeling bcl-6 with purified ab172610 at 1:1500 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

    • Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
      Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
      Anti-Bcl6 antibody [EPR11410-43] (ab172610) at 1/2000 dilution (Purified) + Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 78 kDa
      Observed band size: 78 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)

      Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.

    • Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
      Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
      All lanes : Anti-Bcl6 antibody [EPR11410-43] (ab172610) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 78 kDa



      This image was produced using unpurified antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)This image is courtesy of an anonymous Abreview

      ab172610 staining bcl-6 in Human tonsil tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with 1% PB ab64226 for 10 minutes at room tempreture and antigen retrieval was by heat mediation in EDTA pH 9. The sample was incubated with primary antibody (1/100 in Leica Bond Diluent) at for 30 minutes. A HRP-conjugated Goat anti-rabbit polyclonal (undiluted) was used as the secondary antibody. This image was produced using unpurified antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)

      Immunohistochemical analysis of paraffin-embedded Human B cell lymphoma tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.

    • Anti-Bcl6 antibody [EPR11410-43] (ab172610)
      Anti-Bcl6 antibody [EPR11410-43] (ab172610)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (8)

    Publishing research using ab172610? Please let us know so that we can cite the reference in this datasheet.

    ab172610 has been referenced in 8 publications.

    • Suzuki M  et al. Utility of a Reverse Phase Protein Array to Evaluate Multiple Biomarkers in Diffuse Large B-Cell Lymphoma. Proteomics Clin Appl 14:e1900091 (2020). PubMed: 31721454
    • Khaleefah MM  et al. Isolated oculomotor nerve palsy as a manifestation of diffuse large B cell lymphoma: A case report. Oncol Lett 20:285 (2020). PubMed: 33014163
    • Li N  et al. Pyruvate Kinase M2 Knockdown Suppresses Migration, Invasion, and Epithelial-Mesenchymal Transition of Gastric Carcinoma via Hypoxia-Inducible Factor Alpha/B-Cell Lymphoma 6 Pathway. Biomed Res Int 2020:7467104 (2020). PubMed: 33376737
    • Dheilly E  et al. Cathepsin S Regulates Antigen Processing and T Cell Activity in Non-Hodgkin Lymphoma. Cancer Cell 37:674-689.e12 (2020). PubMed: 32330455
    • Wang K  et al. MicroRNA-155 promotes neointimal hyperplasia through smooth muscle-like cell-derived RANTES in arteriovenous fistulas. J Vasc Surg 67:933-944.e3 (2018). PubMed: 29477204
    • Song Y  et al. Increased frequency of thymic T follicular helper cells in myasthenia gravis patients with thymoma. J Thorac Dis 8:314-22 (2016). IHC ; Human . PubMed: 27076925
    • Sun Y  et al. MicroRNA-155 Promotes the Directional Migration of Resident Smooth Muscle Progenitor Cells by Regulating Monocyte Chemoattractant Protein 1 in Transplant Arteriosclerosis. Arterioscler Thromb Vasc Biol 36:1230-9 (2016). PubMed: 27079875
    • Meyerholz DK  et al. Immunohistochemical Detection of Markers for Translational Studies of Lung Disease in Pigs and Humans. Toxicol Pathol 44:434-41 (2016). IHC-P ; Pig . PubMed: 26511846

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-bcl-6 antibody [EPR11410-43]

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    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    Submitted Feb 09 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-bcl-6 antibody [EPR11410-43]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 08 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-bcl-6 antibody [EPR11410-43]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 19 2017

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