Recombinant Anti-Bcl6 antibody [EPR11410-43] (ab172610)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.com/ps/products/172/ab172610/Images/ab172610-307086-anti-bcl-6-antibody-epr11410-43-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11410-43] to Bcl6
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Bcl6 antibody [EPR11410-43]
See all Bcl6 primary antibodies -
Description
Rabbit monoclonal [EPR11410-43] to Bcl6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human bcl-6 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P41182 -
Positive control
- Ramos, Daudi and Raji cell lysates; Human lymph node, tonsil and B cell lymphoma tissues; Ramos cells.
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General notes
We tested the antibody in mouse and rat samples and tests were unsuccessful therefore we can no longer guaranteed the reactivity of this species.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11410-43 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 87-98 kDa (predicted molecular weight: 78 kDa).
|
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| IHC-P | (3) |
1/1500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/200 - 1/500. See IHC antigen retrieval protocols. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 87-98 kDa (predicted molecular weight: 78 kDa). |
|
IHC-P
1/1500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/200 - 1/500. See IHC antigen retrieval protocols. |
Target
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Function
Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis. -
Tissue specificity
Expressed in germinal center T and B cells and in primary immature dendritic cells. -
Involvement in disease
Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 6 C2H2-type zinc fingers. -
Domain
The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2. -
Post-translational
modificationsPhosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 604 Human
- Omim: 109565 Human
- SwissProt: P41182 Human
- Unigene: 478588 Human
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Alternative names
- B cell CLL/lymphoma 6 antibody
- B cell lymphoma 6 protein antibody
- B-cell lymphoma 5 protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node in stomach tissue sections labeling bcl-6 with purified ab172610 at 1:1500 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.com/ps/products/172/ab172610/Images/ab172610-307070-anti-bcl-6-antibody-epr11410-43-western-blot.jpg)
Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)Anti-Bcl6 antibody [EPR11410-43] (ab172610) at 1/2000 dilution (Purified) + Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 78 kDa
Observed band size: 78 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.com/ps/products/172/ab172610/Images/ab172610-195852-anti-bcl-6-antibody-epr11410-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
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![Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.com/ps/products/172/ab172610/Images/ab172610-195851-anti-bcl-6-antibody-epr11410-43-western-blot.jpg)
Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)All lanes : Anti-Bcl6 antibody [EPR11410-43] (ab172610) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 78 kDaThis image was produced using unpurified antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.com/ps/products/172/ab172610/Images/ab172610-195853-anti-bcl-6-antibody-epr11410-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.com/ps/products/172/ab172610/Images/ab172610-301443-anti-bcl-6-antibody-epr11410-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)This image is courtesy of an anonymous Abreviewab172610 staining bcl-6 in Human tonsil tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with 1% PB ab64226 for 10 minutes at room tempreture and antigen retrieval was by heat mediation in EDTA pH 9. The sample was incubated with primary antibody (1/100 in Leica Bond Diluent) at for 30 minutes. A HRP-conjugated Goat anti-rabbit polyclonal (undiluted) was used as the secondary antibody. This image was produced using unpurified antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.com/ps/products/172/ab172610/Images/ab172610-195854-anti-bcl-6-antibody-epr11410-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)Immunohistochemical analysis of paraffin-embedded Human B cell lymphoma tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
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![Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.com/ps/products/172/ab172610/Images/ab172610-9-benefits-of-recombinant-antibodies.png)
Anti-Bcl6 antibody [EPR11410-43] (ab172610)
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab172610 has been referenced in 6 publications.
- Suzuki M et al. Utility of a Reverse Phase Protein Array to Evaluate Multiple Biomarkers in Diffuse Large B-Cell Lymphoma. Proteomics Clin Appl 14:e1900091 (2020). PubMed: 31721454
- Khaleefah MM et al. Isolated oculomotor nerve palsy as a manifestation of diffuse large B cell lymphoma: A case report. Oncol Lett 20:285 (2020). PubMed: 33014163
- Wang K et al. MicroRNA-155 promotes neointimal hyperplasia through smooth muscle-like cell-derived RANTES in arteriovenous fistulas. J Vasc Surg 67:933-944.e3 (2018). PubMed: 29477204
- Song Y et al. Increased frequency of thymic T follicular helper cells in myasthenia gravis patients with thymoma. J Thorac Dis 8:314-22 (2016). IHC ; Human . PubMed: 27076925
- Sun Y et al. MicroRNA-155 Promotes the Directional Migration of Resident Smooth Muscle Progenitor Cells by Regulating Monocyte Chemoattractant Protein 1 in Transplant Arteriosclerosis. Arterioscler Thromb Vasc Biol 36:1230-9 (2016). PubMed: 27079875
- Meyerholz DK et al. Immunohistochemical Detection of Markers for Translational Studies of Lung Disease in Pigs and Humans. Toxicol Pathol 44:434-41 (2016). IHC-P ; Pig . PubMed: 26511846
