Overview

  • Product name

    Anti-Bcl6 antibody [SP155] - BSA and Azide free
    See all Bcl6 primary antibodies
  • Description

    Rabbit monoclonal [SP155] to Bcl6 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Bcl6 aa 400-500 (C terminal). The exact sequence is proprietary.
    Database link: P41182

  • Positive control

    • IHC-P: Human tonsil, B-cell lymphoma, HK lymphoma, and follicular lymphoma tissue.
  • General notes

    Ab243920 is the carrier-free version of ab183308. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab243920 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab243920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 79 kDa.

Target

  • Function

    Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis.
  • Tissue specificity

    Expressed in germinal center T and B cells and in primary immature dendritic cells.
  • Involvement in disease

    Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
    Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
    Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF.
  • Sequence similarities

    Contains 1 BTB (POZ) domain.
    Contains 6 C2H2-type zinc fingers.
  • Domain

    The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2.
  • Post-translational
    modifications

    Phosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell CLL/lymphoma 6 antibody
    • B cell lymphoma 6 protein antibody
    • B-cell lymphoma 5 protein antibody
    • B-cell lymphoma 6 protein antibody
    • BCL 5 antibody
    • Bcl 6 antibody
    • BCL-5 antibody
    • BCL-6 antibody
    • BCL5 antibody
    • BCL6 antibody
    • BCL6_HUMAN antibody
    • BCL6A antibody
    • cys his2 zinc finger transcription factor antibody
    • cys-his2 zinc finger transcription factor antibody
    • LAZ 3 antibody
    • LAZ 3 protein antibody
    • LAZ3 antibody
    • Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) antibody
    • Lymphoma associated zinc finger gene on chromosome 3 antibody
    • Protein LAZ-3 antibody
    • ZBTB 27 antibody
    • ZBTB27 antibody
    • Zinc finger and BTB domain containing protein 27 antibody
    • Zinc finger and BTB domain-containing protein 27 (ZBTB27) antibody
    • Zinc finger and BTB domain-containing protein 27 antibody
    • Zinc Finger Protein 51 (ZNF51) antibody
    • Zinc finger protein 51 antibody
    • zinc finger transcription factor BCL6S antibody
    • ZNF 51 antibody
    • ZNF51 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Bcl6 with ab183308 at 1/100 dilution (1.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183308)
  • Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183308).

  • Immunohistochemical analysis of paraffin-embedded Human follicular lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183308).

  • Immunohistochemical analysis of paraffin-embedded Human HK lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183308).

  • Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183308).

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183308).

  • Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183308).

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183308).

  • Flow cytometry analysis of Human Ramos cells labeling Bcl6 (green), using ab183308, and negative control of Rabbit IgG (blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183308).

References

ab243920 has not yet been referenced specifically in any publications.

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