Recombinant Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP155] to Bcl6 - C-terminal
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Bcl6 antibody [SP155] - C-terminal
See all Bcl6 primary antibodies -
Description
Rabbit monoclonal [SP155] to Bcl6 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, B-cell lymphoma, HK lymphoma, and follicular lymphoma tissue. WB: Ramos cell lysate. Flow Cyt: Human ramos cell.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP155 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
Use a concentration of 0.135 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 79 kDa).
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IHC-P | (2) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 0.135 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 79 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis. -
Tissue specificity
Expressed in germinal center T and B cells and in primary immature dendritic cells. -
Involvement in disease
Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 6 C2H2-type zinc fingers. -
Domain
The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2. -
Post-translational
modificationsPhosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 604 Human
- Entrez Gene: 12053 Mouse
- Entrez Gene: 303836 Rat
- Omim: 109565 Human
- SwissProt: P41182 Human
- SwissProt: P41183 Mouse
- Unigene: 478588 Human
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Alternative names
- B cell CLL/lymphoma 6 antibody
- B cell lymphoma 6 protein antibody
- B-cell lymphoma 5 protein antibody
see all
Images
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All lanes : Anti-Bcl6 antibody [SP155] - C-terminal (ab183308) at 0.135 µg/ml
Lane 1 : Ramos(Human Burkitt's lymphoma B lymphocyte)
Lane 2 : Raji( Human Burkitt's lymphoma B lymphocyte)
Lane 3 : Daudi( Human Burkitt's lymphoma lymphoblast)
Lane 4 : HEK-293( Human embryonic kidney epithelial cell)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml ("Goat Anti-Rabbit IgG, (H+L),
Peroxidase conjugated ")
Predicted band size: 79 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Bcl6 with ab183308 at 1/100 dilution (1.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human follicular lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human HK lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Bcl6 using ab183308 at a 1/100 dilution.
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Flow cytometry analysis of Human Ramos cells labeling Bcl6 (green), using ab183308, and negative control of Rabbit IgG (blue)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab183308 has been referenced in 1 publication.
- Wang WF et al. HSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cells. Nat Commun 8:363 (2017). PubMed: 28842558