Product nameAnti-BCL9 antibody
DescriptionRabbit polyclonal to BCL9
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human BCL9 aa 800-900 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following cell lysates: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate; Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate; MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate; Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab37305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 140 kDa).Can be blocked with Human BCL9 peptide (ab37304).|
|IHC-P||Use at an assay dependent concentration. PubMed: 22914623|
|ICC/IF||Use a concentration of 1 µg/ml.|
RelevanceBCL9 is involved in signal transduction through the Wnt pathway. It promotes beta-catenin's transcriptional activity. BCL9 is associated with B-cell acute lymphoblastic leukemia. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies (referenced from swissprot and entrez gene).
- B-cell CLL/lymphoma 9 antibody
- BCL 9 antibody
- Legless homolog antibody
- LGS antibody
ab37305 stained in HeLa cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37305 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
All lanes : Anti-BCL9 antibody (ab37305) at 2 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 :
Jurkat nuclear extract lysate (ab14844)
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 140 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Immunohistochemical analysis of Human colorectal cancer tissue, staining BCL9 with ab37305 at 5 µg/ml.
Left panel: BCL9 antibody alone. Right panel: BCL9 antibody + ab37304 - BCL9 peptide.
This product has been referenced in:
- Salazar VS et al. Reactivation of a developmental Bmp2 signaling center is required for therapeutic control of the murine periosteal niche. Elife 8:N/A (2019). Read more (PubMed: 30735122) »
- Wang L et al. Mechanism of Low Expression of miR-30a-5p on Epithelial-Mesenchymal Transition and Metastasis in Ovarian Cancer. DNA Cell Biol 38:341-351 (2019). Read more (PubMed: 30839226) »