Anti-BCMA antibody [1D12G9] (ab233745)
Key features and details
- Mouse monoclonal [1D12G9] to BCMA
- Suitable for: ELISA, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-BCMA antibody [1D12G9]
See all BCMA primary antibodies -
Description
Mouse monoclonal [1D12G9] to BCMA -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human BCMA aa 1-100. Expressed in E.coli.
Database link: Q02223 -
Positive control
- WB: Human BCMA (aa 1-184)-hIgGFc-transfected HEK-293 cell lysate. Flow: K562 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1D12G9 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
1/10000.
|
|
WB |
1/500 - 1/2000. Predicted molecular weight: 20 kDa.
|
|
Flow Cyt |
1/200 - 1/400.
|
Notes |
---|
ELISA
1/10000. |
WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa. |
Flow Cyt
1/200 - 1/400. |
Target
-
Function
Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. -
Tissue specificity
Expressed in mature B-cells, but not in T-cells or monocytes. -
Involvement in disease
Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2. -
Sequence similarities
Contains 1 TNFR-Cys repeat. -
Cellular localization
Cell membrane. Endomembrane system. Perinuclear Golgi-like structures. - Information by UniProt
-
Database links
- Entrez Gene: 608 Human
- Omim: 109545 Human
- SwissProt: Q02223 Human
- Unigene: 2556 Human
-
Alternative names
- B cell maturation antigen antibody
- B cell maturation factor antibody
- B cell maturation protein antibody
see all
Images
-
All lanes : Anti-BCMA antibody [1D12G9] (ab233745) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : BCMA (AA: 1-184)-hIgGFc transfected HEK-293 cell lysate.
Predicted band size: 20 kDa -
Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling BCMA using ab233745 at 1/200 dilution (green) compared with a negative control (red).
-
ELISA showing primary antibody ab233745 binding to the BCMA antigen. Primary antibody was used in a serial dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab233745 has not yet been referenced specifically in any publications.