Recombinant
RabMAb

Recombinant Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

Overview

  • Product name

    Anti-BCMA antibody [EPRBOB-R1-F1-24]
    See all BCMA primary antibodies
  • Description

    Rabbit monoclonal [EPRBOB-R1-F1-24] to BCMA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human BCMA aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q02223

  • Positive control

    • ICC/IF: U266B1 cells. WB: U266B1 whole cell lysate. IP: U266B1 whole cell lysate. Flow Cyt: U266B1 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab245940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 20 kDa.
ICC/IF 1/100.
Flow Cyt 1/60.
IP 1/30.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
    • Tissue specificity

      Expressed in mature B-cells, but not in T-cells or monocytes.
    • Involvement in disease

      Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
    • Sequence similarities

      Contains 1 TNFR-Cys repeat.
    • Cellular localization

      Cell membrane. Endomembrane system. Perinuclear Golgi-like structures.
    • Information by UniProt
    • Database links

    • Alternative names

      • B cell maturation antigen antibody
      • B cell maturation factor antibody
      • B cell maturation protein antibody
      • B-cell maturation protein antibody
      • BCM antibody
      • BCMA antibody
      • CD269 antibody
      • CD269 antigen antibody
      • TNFRSF17 antibody
      • TNR17_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 17 antibody
      see all

    Images

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U266B1 (human multiple myeloma B lymphocyte) cells labeling BCMA with ab245940 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in U266B1 cell line. Negative control cells: Jurkat (PMID: 11104810. The nuclear counterstain is DAPI (blue).
      Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

    • All lanes : Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940) at 1/300 dilution

      Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : U266B1 (human multiple myeloma B lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 20 kDa
      Observed band size: 19,23-30 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23776238).

      Negative control: Jurkat (PMID: 11104810).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • BCMA was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) whole cell lysate with ab245940 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245940 at 1/300 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

      Lane 1: U266B1 whole cell lysate 10 µg (Input).
      Lane 2: ab245940 IP in U266B1 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245940 in U266B1 whole cell lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 30 seconds.

    • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized U266B1 (human multiple myeloma B lymphocyte) cell line labeling BCMA with ab245940 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    References

    ab245940 has not yet been referenced specifically in any publications.

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