Anti-BCMA antibody [Vicky-1] (ab17323)


  • Product name
    Anti-BCMA antibody [Vicky-1]
    See all BCMA primary antibodies
  • Description
    Rat monoclonal [Vicky-1] to BCMA
  • Host species
  • Specificity
    Recognizes human BCMA.
  • Tested applications
    Suitable for: ICC, Flow Cyt, Blockingmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Recombinant human BCMA:Fc. The extracellular domain of human BCMA (amino acids 2-54) is fused to the Fc portion of human IgG1.

  • Positive control
    • U266 cells



Our Abpromise guarantee covers the use of ab17323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

(see recommended protocol and figures).

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

Blocking Use at an assay dependent concentration.

Functional Application (blocks binding of BAFF to human BMCA). 


  • Function
    Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
  • Tissue specificity
    Expressed in mature B-cells, but not in T-cells or monocytes.
  • Involvement in disease
    Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
  • Sequence similarities
    Contains 1 TNFR-Cys repeat.
  • Cellular localization
    Cell membrane. Endomembrane system. Perinuclear Golgi-like structures.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell maturation antigen antibody
    • B cell maturation factor antibody
    • B cell maturation protein antibody
    • B-cell maturation protein antibody
    • BCM antibody
    • BCMA antibody
    • CD269 antibody
    • CD269 antigen antibody
    • TNFRSF17 antibody
    • TNR17_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 17 antibody
    see all


  • Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel).
    Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5min at RT. Slides were blocked with normal IgG, and incubated for 1 hour with 5µg/ml ab17323 in 1%BSA / 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

  • FACS analysis of cells with ab17323 to BCMA.

    Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human BCMA. Cells (5x105) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of ab17323 monoclonal antibody to human BCMA. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry.

  • Detection of endogenous human BCMA with ab17323 to BCMA (human)(Vicky-1) (second peak, to the right). Method: U266 cells (2x105) were incubated on ice for 30 minutes with 0.2 µg of ab17323 or an isotype control in 25µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide). The primary antibody was revealed with anti- Rat IgG (R-PE) and then analyzed by Flow Cytometry.


ab17323 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. I am sorry to confirm that as far as we are aware, ab17323 has never been tested with samples from cynomolgus monkey. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these a...

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I'm sorry to hear the customer is experiencing problems in FACS with some of our antibodies, it is likely that the problem is protocol related. I have received this morning a detailed FACS protocol with details of the buffers to use and fixative to use...

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The antibody to BCMA (Vicky-1) has been tested by FACS analysis on several cells (B cells from Myeloma U266 or in B cell Lymphoma suDHL-6). The endogenous protein is well detected. If you have any additional questions, please contact us again.


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