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Recombinant human BCMA:Fc. The extracellular domain of human BCMA (amino acids 2-54) is fused to the Fc portion of human IgG1.
Our Abpromise guarantee covers the use of ab17323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use a concentration of 1 µg/ml.
(see recommended protocol and figures).
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Blocking||Use at an assay dependent concentration.
Functional Application (blocks binding of BAFF to human BMCA).
Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel).
Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5min at RT. Slides were blocked with normal IgG, and incubated for 1 hour with 5
FACS analysis of cells with ab17323 to BCMA.
Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human BCMA. Cells (5x105) were incubated on ice for 30 min in 50
ab17323 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"