Overview

  • Product name

  • Description

    Rabbit polyclonal to BCOR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 1115-1144 of Human BCOR.

  • Positive control

    • 293 cell line and Mouse stomach tissue lysates; Mouse brain tissue; 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab135801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 192 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor. Involved in the repression of TFAP2A; impairs binding of BCL6 and KDM2B to TFAP2A promoter regions. Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem cells; the function implies inhibition of methylation on histone H3 'Lys-4' (H3K4me3) and 'Lys-36' (H3K36me2).
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Defects in BCOR are the cause of microphthalmia syndromic type 2 (MCOPS2) [MIM:300166]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS2 is a very rare multiple congenital anomaly syndrome characterized by eye anomalies (congenital cataract, microphthalmia, or secondary glaucoma), facial abnormalities (long narrow face, high nasal bridge, pointed nose with cartilages separated at the tip, cleft palate, or submucous cleft palate), cardiac anomalies (atrial septal defect, ventricular septal defect, or floppy mitral valve) and dental abnormalities (canine radiculomegaly, delayed dentition, oligodontia, persistent primary teeth, or variable root length).
  • Sequence similarities

    Belongs to the BCOR family.
    Contains 3 ANK repeats.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5830466J11Rik antibody
    • 8430401K06Rik antibody
    • ANOP 2 antibody
    • ANOP2 antibody
    • BCL 6 co repressor antibody
    • BCL 6 corepressor antibody
    • BCL 6 interacting corepressor antibody
    • BCL-6 corepressor antibody
    • BCL6 co repressor antibody
    • BCL6 corepressor antibody
    • BCL6 interacting corepressor antibody
    • BCoR antibody
    • BCOR_HUMAN antibody
    • FLJ20285 antibody
    • FLJ38041 antibody
    • KIAA1575 antibody
    • MAA 2 antibody
    • MAA2 antibody
    • MCOPS 2 antibody
    • MCOPS2 antibody
    • MGC131961 antibody
    • MGC71031 antibody
    see all

Images

  • Anti-BCOR antibody (ab135801) at 1/50 dilution + 293 cell line lysate at 35 µg

    Predicted band size: 192 kDa

  • Anti-BCOR antibody (ab135801) at 1/50 dilution + Mouse stomach tissue lysate at 35 µg

    Predicted band size: 192 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse brain tissue labelling BCOR with ab135801 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
  • Flow Cytometric analysis of 293 cells labelling BCOR with ab135801 at 1/10 dilution (green) compared to a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:

  • Wong MK  et al. Clear cell sarcomas of the kidney are characterised by BCOR gene abnormalities, including exon 15 internal tandem duplications and BCOR-CCNB3 gene fusion. Histopathology 72:320-329 (2018). Read more (PubMed: 28833375) »
See 1 Publication for this product

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