Overview

  • Product name

  • Description

    Rabbit polyclonal to BCOR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to internal sequence amino acids of Human BCOR.

  • Positive control

    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab135975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 192 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor. Involved in the repression of TFAP2A; impairs binding of BCL6 and KDM2B to TFAP2A promoter regions. Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem cells; the function implies inhibition of methylation on histone H3 'Lys-4' (H3K4me3) and 'Lys-36' (H3K36me2).
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Defects in BCOR are the cause of microphthalmia syndromic type 2 (MCOPS2) [MIM:300166]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS2 is a very rare multiple congenital anomaly syndrome characterized by eye anomalies (congenital cataract, microphthalmia, or secondary glaucoma), facial abnormalities (long narrow face, high nasal bridge, pointed nose with cartilages separated at the tip, cleft palate, or submucous cleft palate), cardiac anomalies (atrial septal defect, ventricular septal defect, or floppy mitral valve) and dental abnormalities (canine radiculomegaly, delayed dentition, oligodontia, persistent primary teeth, or variable root length).
  • Sequence similarities

    Belongs to the BCOR family.
    Contains 3 ANK repeats.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5830466J11Rik antibody
    • 8430401K06Rik antibody
    • ANOP 2 antibody
    • ANOP2 antibody
    • BCL 6 co repressor antibody
    • BCL 6 corepressor antibody
    • BCL 6 interacting corepressor antibody
    • BCL-6 corepressor antibody
    • BCL6 co repressor antibody
    • BCL6 corepressor antibody
    • BCL6 interacting corepressor antibody
    • BCoR antibody
    • BCOR_HUMAN antibody
    • FLJ20285 antibody
    • FLJ38041 antibody
    • KIAA1575 antibody
    • MAA 2 antibody
    • MAA2 antibody
    • MCOPS 2 antibody
    • MCOPS2 antibody
    • MGC131961 antibody
    • MGC71031 antibody
    see all

Images

  • Anti-BCOR antibody (ab135975) at 1/500 dilution + HeLa cell lysate at 30 µg

    Predicted band size: 192 kDa

References

ab135975 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
Specification
Mouse embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 13 2015

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