Key features and details
- Mouse polyclonal to BCOR
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BCOR antibody
See all BCOR primary antibodies
DescriptionMouse polyclonal to BCOR
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length Human BCOR (short isoform, NP_065977.1)
- Human colon tissue lysate. HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 107 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionTranscriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor. Involved in the repression of TFAP2A; impairs binding of BCL6 and KDM2B to TFAP2A promoter regions. Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem cells; the function implies inhibition of methylation on histone H3 'Lys-4' (H3K4me3) and 'Lys-36' (H3K36me2).
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in BCOR are the cause of microphthalmia syndromic type 2 (MCOPS2) [MIM:300166]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS2 is a very rare multiple congenital anomaly syndrome characterized by eye anomalies (congenital cataract, microphthalmia, or secondary glaucoma), facial abnormalities (long narrow face, high nasal bridge, pointed nose with cartilages separated at the tip, cleft palate, or submucous cleft palate), cardiac anomalies (atrial septal defect, ventricular septal defect, or floppy mitral valve) and dental abnormalities (canine radiculomegaly, delayed dentition, oligodontia, persistent primary teeth, or variable root length).
Sequence similaritiesBelongs to the BCOR family.
Contains 3 ANK repeats.
- Information by UniProt
- 5830466J11Rik antibody
- 8430401K06Rik antibody
- ANOP 2 antibody
Anti-BCOR antibody (ab88112) at 1 µg/ml + Human colon tissue lysate at 50 µg
Goat anti-Mouse IgG at 1/5000 dilution
Predicted band size: 107 kDa
All lanes : Anti-BCOR antibody (ab88112) at 1 µg/ml
Lane 1 : BCOR-transfected 293T cell lysate
Lane 2 : untransfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-Mouse IgG at 1/5000 dilution
Predicted band size: 107 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Immunofluorescence staining of BCOR in HeLa cells using ab88112 at 10 µg/ml followed by a fluorescein conjugated secondary antibody.
ab88112 has been referenced in 5 publications.
- Vewinger N et al. IGF1R Is a Potential New Therapeutic Target for HGNET-BCOR Brain Tumor Patients. Int J Mol Sci 20:N/A (2019). PubMed: 31234291
- Paret C et al. Activation of the basal cell carcinoma pathway in a patient with CNS HGNET-BCOR diagnosis: consequences for personalized targeted therapy. Oncotarget 7:83378-83391 (2016). WB ; Human . PubMed: 27825128
- Roy A et al. Recurrent internal tandem duplications of BCOR in clear cell sarcoma of the kidney. Nat Commun 6:8891 (2015). Human . PubMed: 26573325
- Yoon J et al. Interferon regulatory factor 8 (IRF8) interacts with the B cell lymphoma 6 (BCL6) corepressor BCOR. J Biol Chem 289:34250-7 (2014). ICC . PubMed: 25331958
- Grossmann V et al. Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype. Blood 118:6153-63 (2011). WB ; Human . PubMed: 22012066