Product nameAnti-Bcr antibody
See all Bcr primary antibodies
DescriptionRabbit polyclonal to Bcr
Tested applicationsSuitable for: IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or WB
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Bcr aa 1250-1350 (C terminal). The exact sequence is proprietary.
- Jurkat cell lysate, human lung carcinoma and HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab32523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250.
Is unsuitable for FACS or WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionGTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Involvement in diseaseNote=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
Sequence similaritiesContains 1 C2 domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
DomainThe region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
The DH domain is involved in interaction with CCPG1.
modificationsAutophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
- Information by UniProt
- ALL antibody
- bcr antibody
- BCR/ABL FUSION GENE, INCLUDED antibody
Anti-Bcr antibody (ab32523) at 1/40 dilution + immunoprecipitation pellets from Jurkat cell lysate
Observed band size: 130 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ab32523 at 1/100 dilution.
Immunofluorescent staining of HeLa cells using ab32523 at 1/250 dilution.
ab32523 has not yet been referenced specifically in any publications.