• Product name

  • Description

    Rabbit polyclonal to Bcr
  • Host species

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IF, ICCmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Medaka fish, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1221 and 1271 of human Bcr using the numbering given in entry (NP_004318.3).

  • Positive control

    • HeLa whole cell lysate 293T whole cell lysate K562 cells



Our Abpromise guarantee covers the use of ab86173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
IP Use at 10 µg/mg of lysate.
Flow Cyt Use 0.5µg for 106 cells.

(in 150µl).




ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


ICC/IF Use a concentration of 5 µg/ml.
ICC 1/500 - 1/2000.

Antigen retrieval with citrate buffer pH6.0 is recommended for formalin-fixed paraffin-embedded cells. For cytospin preparations of formaldehyde fixed cells permeabilization with Triton-X 100 is recommended.


  • Function

    GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
  • Involvement in disease

    Note=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
  • Domain

    The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
    The DH domain is involved in interaction with CCPG1.
  • Post-translational

    Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALL antibody
    • bcr antibody
    • BCR/FGFR1 chimera protein antibody
    • BCR_HUMAN antibody
    • BCR1 antibody
    • Breakpoint cluster region antibody
    • Breakpoint cluster region protein antibody
    • CML antibody
    • D22S11 antibody
    • D22S662 antibody
    • FGFR1/BCR chimera protein antibody
    • PHL antibody
    • Renal carcinoma antigen NY-REN-26 antibody
    see all


  • All lanes : Anti-Bcr antibody (ab86173) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 143 kDa

    Exposure time: 30 seconds
  • Immunocytochemistry/Immunofluorescence analysis of human K562 cells labelling Bcr with ab86173 at 1/1000 (1µg/ml). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
  • Detection of Human Bcr by Immunoprecipitation, using ab86173 at 10µg/mg lysate. Image shows immunoprecipitated Bcr detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with an antibody recognising an upstream epitope in lane 1, ab86173 at 1 µg/ml in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 10 seconds.
  • Flow Cytometric Detection of Bcr. 1 x 106 K562 cells were fixed, permeabilized, and stained with ab86173 at 0.25 µg in a 150 µl reaction. Image shows Isotype anti-KLH control (red), no antibody (blue) and ab86173 (black).
  • ICC/IF image of ab86173 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86173, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab86173 has not yet been referenced specifically in any publications.

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