Key features and details
- Rabbit polyclonal to Bcr
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Bcr antibody
See all Bcr primary antibodies
DescriptionRabbit polyclonal to Bcr
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla
Synthetic peptide, corresponding to a region between residue 50 and 100 of human Bcr (NP_004318.3)
- human K562 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab87269 was affinity purified using an epitope specific to Bcr immobilized on solid support.
Our Abpromise guarantee covers the use of ab87269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/500. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionGTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Involvement in diseaseNote=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
Sequence similaritiesContains 1 C2 domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
DomainThe region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
The DH domain is involved in interaction with CCPG1.
modificationsAutophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
- Information by UniProt
- ALL antibody
- bcr antibody
- BCR/ABL FUSION GENE, INCLUDED antibody
ab87269 staining Bcr in Human B cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.5% Triton X-100 in PBS and blocked with 1% BSA for 90 minutes at 20°C. Samples were incubated with primary antibody (1/50; 1% BSA in PBS) for 2 hours 30 minutes at 20°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody
ab87269 at 1/250 dilution staining Bcr in human K562 cells by Immunofluorescence, Formalin-fixed, Paraffin-embedded tissue. Detection: Red fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
ab87269 at 1/250 dilution staining Bcr in K562 cells by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
ab87269 has not yet been referenced specifically in any publications.