Overview

  • Product name

    Anti-Bcr antibody - C-terminal
    See all Bcr primary antibodies
  • Description

    Rabbit polyclonal to Bcr - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment within Human Bcr (C terminal). The exact sequence is proprietary.
    Database link: P11274

  • Positive control

    • IHC-P: H441 xenograft tissue. WB: MOLT-4 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 143 kDa.

Target

  • Function

    GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
  • Involvement in disease

    Note=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
  • Domain

    The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
    The DH domain is involved in interaction with CCPG1.
  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALL antibody
    • bcr antibody
    • BCR/ABL FUSION GENE, INCLUDED antibody
    • BCR/FGFR1 chimera protein antibody
    • BCR/FGFR1 FUSION GENE, INCLUDED antibody
    • BCR/PDGFRA FUSION GENE, INCLUDED antibody
    • BCR_HUMAN antibody
    • BCR1 antibody
    • Breakpoint cluster region antibody
    • Breakpoint cluster region protein antibody
    • CML antibody
    • D22S11 antibody
    • D22S662 antibody
    • FGFR1/BCR chimera protein antibody
    • PHL antibody
    • Renal carcinoma antigen NY-REN-26 antibody
    see all

Images

  • Anti-Bcr antibody - C-terminal (ab227186) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg

    Predicted band size: 143 kDa



    5% SDS-PAGE gel.

  • Paraffin-embedded H441 xenograft tissue stained for Bcr using ab227186 at 1/500 dilution in immunohistochemical analysis.

References

ab227186 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227186.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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