Key features and details
- Rabbit polyclonal to Bcr - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Bcr antibody - C-terminal
See all Bcr primary antibodies
DescriptionRabbit polyclonal to Bcr - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment within Human Bcr (C terminal). The exact sequence is proprietary.
Database link: P11274
- IHC-P: H441 xenograft tissue. WB: MOLT-4 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 143 kDa.|
FunctionGTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Involvement in diseaseNote=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
Sequence similaritiesContains 1 C2 domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
DomainThe region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
The DH domain is involved in interaction with CCPG1.
modificationsAutophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
- Information by UniProt
- ALL antibody
- bcr antibody
- BCR/ABL FUSION GENE, INCLUDED antibody
Anti-Bcr antibody - C-terminal (ab227186) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 143 kDa
5% SDS-PAGE gel.
Paraffin-embedded H441 xenograft tissue stained for Bcr using ab227186 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227186 has not yet been referenced specifically in any publications.