Recombinant
RabMAb

Recombinant Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287)

Submit a review Submit a question

Overview

  • Product name

    Anti-Bcr antibody [EP535Y] - BSA and Azide free
    See all Bcr primary antibodies

  • Description

    Rabbit monoclonal [EP535Y] to Bcr - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Bcr aa 1250-1350 (C terminal). The exact sequence is proprietary.

  • General notes

    ab247287 is the carrier-free version of ab40779 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247287 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.

  • Involvement in disease

    Note=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).

  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.

  • Domain

    The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
    The DH domain is involved in interaction with CCPG1.

  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
  • Information by UniProt

  • Database links

    • Alternative names

      • ALL antibody
      • bcr antibody
      • BCR/ABL FUSION GENE, INCLUDED antibody
      • BCR/FGFR1 chimera protein antibody
      • BCR/FGFR1 FUSION GENE, INCLUDED antibody
      • BCR/PDGFRA FUSION GENE, INCLUDED antibody
      • BCR_HUMAN antibody
      • BCR1 antibody
      • Breakpoint cluster region antibody
      • Breakpoint cluster region protein antibody
      • CML antibody
      • D22S11 antibody
      • D22S662 antibody
      • FGFR1/BCR chimera protein antibody
      • PHL antibody
      • Renal carcinoma antigen NY-REN-26 antibody
      see all

    References

    ab247287 has not yet been referenced specifically in any publications.

    Publishing research using ab247287? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab247287.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com