Recombinant Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287)
![Western blot - Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287)](https://www.abcam.com/ps/products/247/ab247287/Images/ab247287-415231-anti-bcr-antibody-ep535y-westernblot-k-562-human.jpg "Western blot - Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP535Y] to Bcr - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Bcr antibody [EP535Y] - BSA and Azide free
See all Bcr primary antibodies -
Description
Rabbit monoclonal [EP535Y] to Bcr - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Bcr aa 1250-1350 (C terminal). The exact sequence is proprietary.
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Positive control
- K562 cell lysate, human lymphoma, HeLa cells.
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General notes
ab247287 is the carrier-free version of ab40779 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247287 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP535Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa). |
Target
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Function
GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity. -
Involvement in disease
Note=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). -
Sequence similarities
Contains 1 C2 domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 Rho-GAP domain. -
Domain
The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
The DH domain is involved in interaction with CCPG1. -
Post-translational
modificationsAutophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2. - Information by UniProt
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Database links
- Entrez Gene: 613 Human
- Omim: 151410 Human
- SwissProt: P11274 Human
- Unigene: 517461 Human
- Unigene: 715409 Human
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Alternative names
- ALL antibody
- bcr antibody
- BCR/ABL FUSION GENE, INCLUDED antibody
see all
Images
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Western blot - Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287)Anti-Bcr antibody [EP535Y] (ab40779) at 1/1000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 143 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This data was developed using ab40779, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287)](https://www.abcam.com/ps/products/247/ab247287/Images/ab247287-2-anti-bcr-antibody-ep535y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287) -
![Immunocytochemistry - Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287)](https://www.abcam.com/ps/products/247/ab247287/Images/ab247287-1-anti-bcr-antibody-ep535y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287) -
![Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287)](https://www.abcam.com/ps/products/247/ab247287/Images/ab247287-4-benefits-of-recombinant-antibodies.png)
Anti-Bcr antibody [EP535Y] - BSA and Azide free (ab247287)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247287 has not yet been referenced specifically in any publications.
