Overview

  • Product name

    Anti-Bcr antibody [EPR22062]
    See all Bcr primary antibodies
  • Description

    Rabbit monoclonal [EPR22062] to Bcr
  • Host species

    Rabbit
  • Specificity

    IHC is not recommended for human.
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Bcr aa 150-350. The exact sequence is proprietary.
    Database link: P11274

  • Positive control

    • WB: K562, PC-12, NIH/3T3, MCF7 and HepG2 whole cell lysates; Human and rat brain tissue lysates; Human testis tissue lysate. IHC-P: Mouse hippocampus and rat cerebrum tissue. Flow Cyt: K562 cells. IP: HeLa whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 230, 210, 160, 130 kDa (predicted molecular weight: 143 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
  • Involvement in disease

    Note=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
  • Domain

    The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
    The DH domain is involved in interaction with CCPG1.
  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALL antibody
    • bcr antibody
    • BCR/ABL FUSION GENE, INCLUDED antibody
    • BCR/FGFR1 chimera protein antibody
    • BCR/FGFR1 FUSION GENE, INCLUDED antibody
    • BCR/PDGFRA FUSION GENE, INCLUDED antibody
    • BCR_HUMAN antibody
    • BCR1 antibody
    • Breakpoint cluster region antibody
    • Breakpoint cluster region protein antibody
    • CML antibody
    • D22S11 antibody
    • D22S662 antibody
    • FGFR1/BCR chimera protein antibody
    • PHL antibody
    • Renal carcinoma antigen NY-REN-26 antibody
    see all

Images

  • All lanes : Anti-Bcr antibody [EPR22062] (ab222406) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole lysate
    Lane 2 : Human brain lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 143 kDa
    Observed band size: 130,160 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lanes 1 and 2: 3 minutes; Lanes 3 and 4: 59 seconds; Lane 3: 81 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The 160 kDa and 130 kDa bands are isoforms of BCR (PMID: 2494632, PMID: 3078961).

  • Bcr was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222406 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222406 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection antibody at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10μg (Input).
    Lane 2: ab222406 IP in HeLa whole cell lysate (+)
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222406 in HeLa whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    The 160 kDa and 130 kDa bands are isoforms of BCR (PMID:2494632, PMID:3078961).

     

     

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell line labeling Bcr with ab222406 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Bcr with ab222406 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in rat cerebrum (PMID: 25331951). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Bcr with ab222406 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in mouse hippocampus (PMID: 25331951). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

  • All lanes : Anti-Bcr antibody [EPR22062] (ab222406) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole lysate
    Lane 3 : NIH/3T3 (mouse embyro fibroblast cell line) whole lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 143 kDa
    Observed band size: 130,160,210,230 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The 230/210 kDa band is the BCR-ABL fusion protein in K562 cells (PMID:17684099, PMID:16585609, PMID:12476301). The 160 kDa and 130 kDa bands are isoforms of BCR (PMID:2494632, PMID:3078961).

References

ab222406 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222406.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up