Anti-BCRP/ABCG2 antibody [1H2] (ab130244)
Key features and details
- Mouse monoclonal [1H2] to BCRP/ABCG2
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-BCRP/ABCG2 antibody [1H2]
See all BCRP/ABCG2 primary antibodies -
Description
Mouse monoclonal [1H2] to BCRP/ABCG2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey -
Immunogen
Purified recombinant fragment of Human BCRP/ABCG2 expressed in E. coli.
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Positive control
- HepG2, COS7 and NIH/3T3 cell lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituent: 99.99% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
1H2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 72 kDa.
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ELISA |
1/10000.
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ICC/IF |
1/200 - 1/1000.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 72 kDa. |
ELISA
1/10000. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. -
Tissue specificity
Highly expressed in placenta. Low expression in small intestine, liver and colon. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
Post-translational
modificationsGlycosylation-deficient ABCG2 is normally expressed and functional. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9429 Human
- Entrez Gene: 26357 Mouse
- Omim: 603756 Human
- SwissProt: Q9UNQ0 Human
- SwissProt: Q7TMS5 Mouse
- Unigene: 480218 Human
- Unigene: 333096 Mouse
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Alternative names
- ABC transporter antibody
- ABC15 antibody
- ABCG 2 antibody
see all
Images
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All lanes : Anti-BCRP/ABCG2 antibody [1H2] (ab130244) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : COS7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : NIH/3T3 cell lysate
Predicted band size: 72 kDa -
ab130244, at 1/200 dilution, staining BCRP/ABCG2 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ELISA using ab130244.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab130244 has been referenced in 7 publications.
- Yang H et al. FBI-1 enhanced the resistance of triple-negative breast cancer cells to chemotherapeutic agents via the miR-30c/PXR axis. Cell Death Dis 11:851 (2020). PubMed: 33051436
- Ke B et al. Interleukin-7 Resensitizes Non-Small-Cell Lung Cancer to Cisplatin via Inhibition of ABCG2. Mediators Inflamm 2019:7241418 (2019). PubMed: 31915416
- Cheng CC et al. Stat3/Oct-4/c-Myc signal circuit for regulating stemness-mediated doxorubicin resistance of triple-negative breast cancer cells and inhibitory effects of WP1066. Int J Oncol 53:339-348 (2018). PubMed: 29750424
- Wang P et al. HIF1a regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia. Oncotarget 8:28074-28092 (2017). PubMed: 28427209
- Park SJ et al. Estradiol, TGF-ß1 and hypoxia promote breast cancer stemness and EMT-mediated breast cancer migration. Oncol Lett 11:1895-1902 (2016). PubMed: 26998096
- Topalis D et al. Resistance to the nucleotide analogue cidofovir in HPV(+) cells: a multifactorial process involving UMP/CMP kinase 1. Oncotarget 7:10386-401 (2016). WB . PubMed: 26824416
- Wang C et al. Associations between ABCG2 gene polymorphisms and isolated septal defects in a Han Chinese population. DNA Cell Biol 33:689-98 (2014). PubMed: 24979295