Product nameAnti-BCRP/ABCG2 antibody
See all BCRP/ABCG2 primary antibodies
DescriptionRabbit polyclonal to BCRP/ABCG2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human, Pig
Predicted to work with: Rat, Macaque monkey
Synthetic peptide corresponding to Human BCRP/ABCG2 aa 550-650 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Placenta Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab63907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).|
FunctionXenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Tissue specificityHighly expressed in placenta. Low expression in small intestine, liver and colon.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.
modificationsGlycosylation-deficient ABCG2 is normally expressed and functional.
Cellular localizationCell membrane.
- Information by UniProt
- ABC transporter antibody
- ABC15 antibody
- ABCG 2 antibody
Anti-BCRP/ABCG2 antibody (ab63907) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg
Secondary anti-rabbit antibody at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 10 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This product has been referenced in:
- You F et al. miRNA-106a Promotes Breast Cancer Cell Proliferation, Clonogenicity, Migration, and Invasion through Inhibiting Apoptosis and Chemosensitivity. DNA Cell Biol N/A:N/A (2018). Read more (PubMed: 30570350) »
- Lin J et al. Scutellaria barbata D. Don inhibits 5-fluorouracil resistance in colorectal cancer by regulating PI3K/AKT pathway. Oncol Rep 38:2293-2300 (2017). Read more (PubMed: 28849113) »