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Fusion protein composed of E. Coli maltose binding protein and BCRP peptide [126 amino acids, 271-396 AA of BCRP, GeneBank accession #AF098951].
For the WB, please note the subcellular localization and distribution of the BCRP/ABCG2 in Normal Human Tissues: PMID:11309308
We have received mixed feedback from customers, the antibody showed 45-50 kDa band size in A549 and Caco2 cell lines against predicted size of 72kDa. In response to data we have received we no longer guarantee this antibody for western blot. ABCG2 is a multi-pass membrane protein so for troubleshooting we would recommend heating the samples at 60-70C for 15-20 minutes instead of boiling and as this protein exists as homodimer so complete reduction of samples is highly recommended. In case of any other question please email scientific support team.
Our Abpromise guarantee covers the use of ab3380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/20 - 1/50. Acetone fixed cells.|
|IHC-Fr||1/20 - 1/40.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 24136221
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
Immunohistochemical analysis of human breast cancer tissue, staining BCRP/ABCG2 with ab3380.
Tissue was fixed with formaldehyde, blocked with 3% H2O2 for 5 minutes at 20°C and permeabilized with PBS-Tween; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/200 in PBS-Tween) for 1 hour at 20°C. A biotinylated goat anti-mouse polyclonal IgG was used as the secondary antibody.
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