Anti-BCRP/ABCG2 antibody [BXP-21] (ab3380)

Reviews (11)Q&A (13)References (106)

Key features and details

  • Mouse monoclonal [BXP-21] to BCRP/ABCG2
  • Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, ICC
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-BCRP/ABCG2 antibody [BXP-21]
    See all BCRP/ABCG2 primary antibodies

  • Description

    Mouse monoclonal [BXP-21] to BCRP/ABCG2

  • Host species

    Mouse

  • Specificity

    BXP-21 reacts with an internal epitope of BCRP/ABCG2, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. This antibody does not react with the human MDR1, MRP1 and MRP2 gene products.

  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within BCRP/ABCG2 aa 250-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

  • Positive control

    • IHC-P: Human breast cancer tissue. ICC/IF: MCF7 cells. Caco Cell lysate (see second reference cited).

  • General notes

    For the WB, please note the subcellular localization and distribution of the BCRP/ABCG2 in Normal Human Tissues: PMID:11309308

    We have received mixed feedback from customers, the antibody showed 45-50 kDa band size in A549 and Caco2 cell lines against predicted size of 72kDa. In response to data we have received we no longer guarantee this antibody for western blot. ABCG2 is a multi-pass membrane protein so for troubleshooting we would recommend heating the samples at 60-70C for 15-20 minutes instead of boiling and as this protein exists as homodimer so complete reduction of samples is highly recommended. In case of any other question please email scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
IHC-P (2)
Use at an assay dependent concentration.
ICC/IF (2)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 24136221

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC
Use at an assay dependent concentration.

Acetone fixed cells.
Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 24136221

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC
Use at an assay dependent concentration.

Acetone fixed cells.

Target

  • Function

    Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

  • Tissue specificity

    Highly expressed in placenta. Low expression in small intestine, liver and colon.

  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.

  • Post-translational
    modifications

    Glycosylation-deficient ABCG2 is normally expressed and functional.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ABC transporter antibody
      • ABC15 antibody
      • ABCG 2 antibody
      • ABCG2 antibody
      • ABCG2_HUMAN antibody
      • ABCP antibody
      • ATP binding cassette sub family G (WHITE) member 2 antibody
      • ATP binding cassette transporter G2 antibody
      • ATP-binding cassette sub-family G member 2 antibody
      • BCRP antibody
      • BCRP1 antibody
      • BMDP antibody
      • Breast cancer resistance protein antibody
      • CD338 antibody
      • CDw338 antibody
      • CDw338 antigen antibody
      • EST157481 antibody
      • GOUT1 antibody
      • MGC102821 antibody
      • Mitoxantrone resistance associated protein antibody
      • Mitoxantrone resistance-associated protein antibody
      • MRX antibody
      • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
      • MXR antibody
      • MXR1 antibody
      • Placenta specific ATP binding cassette transporter antibody
      • Placenta specific MDR protein antibody
      • Placenta-specific ATP-binding cassette transporter antibody
      • UAQTL1 antibody
      see all

    References (106)

    Publishing research using ab3380? Please let us know so that we can cite the reference in this datasheet.

    ab3380 has been referenced in 106 publications.

    • Bartos Z & Homolya L Identification of Specific Trafficking Defects of Naturally Occurring Variants of the Human ABCG2 Transporter. Front Cell Dev Biol 9:615729 (2021). PubMed: 33634118
    • Wu CP  et al. Overexpression of Human ABCB1 and ABCG2 Reduces the Susceptibility of Cancer Cells to the Histone Deacetylase 6-Specific Inhibitor Citarinostat. Int J Mol Sci 22:N/A (2021). PubMed: 33807514
    • Zhang J  et al. Rapamycin Antagonizes BCRP-Mediated Drug Resistance Through the PI3K/Akt/mTOR Signaling Pathway in mPRa-Positive Breast Cancer. Front Oncol 11:608570 (2021). PubMed: 33912444
    • Versele R  et al. Ketone Bodies Promote Amyloid-ß1-40 Clearance in a Human in Vitro Blood-Brain Barrier Model. Int J Mol Sci 21:N/A (2020). PubMed: 32023814
    • Chen HD  et al. Novel mutations in BRCA2 intron 11 and overexpression of COX-2 and BIRC3 mediate cellular resistance to PARP inhibitors. Am J Cancer Res 10:2813-2831 (2020). PubMed: 33042619
    View all Publications for this product

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-BCRP/ABCG2 antibody [BXP-21]

     Below Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (Rabbit cornea. Limbal epithelial area. Only progen)
    Specification
    Rabbit cornea. Limbal epithelial area. Only progen
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    DR. Noelia Andollo

    Verified customer

    Submitted Jun 23 2015

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