Anti-BCRP/ABCG2 antibody [BXP-21] (ab3380)
Key features and details
- Mouse monoclonal [BXP-21] to BCRP/ABCG2
- Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-BCRP/ABCG2 antibody [BXP-21]
See all BCRP/ABCG2 primary antibodies -
Description
Mouse monoclonal [BXP-21] to BCRP/ABCG2 -
Host species
Mouse -
Specificity
BXP-21 reacts with an internal epitope of BCRP/ABCG2, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. This antibody does not react with the human MDR1, MRP1 and MRP2 gene products.
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Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within BCRP/ABCG2 aa 250-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- IHC-P: Human breast cancer tissue. ICC/IF: MCF7 cells. Caco Cell lysate (see second reference cited).
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General notes
For the WB, please note the subcellular localization and distribution of the BCRP/ABCG2 in Normal Human Tissues: PMID:11309308
We have received mixed feedback from customers, the antibody showed 45-50 kDa band size in A549 and Caco2 cell lines against predicted size of 72kDa. In response to data we have received we no longer guarantee this antibody for western blot. ABCG2 is a multi-pass membrane protein so for troubleshooting we would recommend heating the samples at 60-70C for 15-20 minutes instead of boiling and as this protein exists as homodimer so complete reduction of samples is highly recommended. In case of any other question please email scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: 0.7% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Primary antibody notes
Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone. -
Clonality
Monoclonal -
Clone number
BXP-21 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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IHC-P | (2) |
Use at an assay dependent concentration.
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ICC/IF | (2) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 24136221
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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ICC |
Use at an assay dependent concentration.
Acetone fixed cells. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 24136221 ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC
Use at an assay dependent concentration. Acetone fixed cells. |
Target
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Function
Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. -
Tissue specificity
Highly expressed in placenta. Low expression in small intestine, liver and colon. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
Post-translational
modificationsGlycosylation-deficient ABCG2 is normally expressed and functional. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9429 Human
- Omim: 603756 Human
- SwissProt: Q9UNQ0 Human
- Unigene: 480218 Human
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Alternative names
- ABC transporter antibody
- ABC15 antibody
- ABCG 2 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (113)
ab3380 has been referenced in 113 publications.
- Wakamatsu K et al. Metabolites and Biomarker Compounds of Neurodegenerative Diseases in Cerebrospinal Fluid. Metabolites 12:N/A (2022). PubMed: 35448530
- Lee S et al. Inhibition of MEK-ERK pathway enhances oncolytic vaccinia virus replication in doxorubicin-resistant ovarian cancer. Mol Ther Oncolytics 25:211-224 (2022). PubMed: 35592390
- Versele R et al. TNF-α and IL-1β Modulate Blood-Brain Barrier Permeability and Decrease Amyloid-β Peptide Efflux in a Human Blood-Brain Barrier Model. Int J Mol Sci 23:N/A (2022). PubMed: 36142143
- Pavlič R et al. Altered Profile of E1-S Transporters in Endometrial Cancer: Lower Protein Levels of ABCG2 and OSTβ and Up-Regulation of SLCO1B3 Expression. Int J Mol Sci 22:N/A (2021). PubMed: 33917029
- Loiola RA et al. Secretome of endothelial progenitor cells from stroke patients promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakage. Stem Cell Res Ther 12:552 (2021). PubMed: 34702368