Product nameAnti-BCRP/ABCG2 antibody [BXP-53]
See all BCRP/ABCG2 primary antibodies
DescriptionRat monoclonal [BXP-53] to BCRP/ABCG2
Tested applicationsSuitable for: ICC, WB, Flow Cyt, IHC-P, ICC/IFmore details
Unsuitable for: EMSA
Species reactivityReacts with: Mouse, Human
Fusion protein containing the E. coli maltose binding protein and a fragment corresponding to amino acids 221-394 of Mouse BCRP/ABCG2
EpitopeReacts with an internal epitope of BCRP/ABCG2.
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab24115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/20 - 1/50. Acetone fixed cytospin.|
|WB||1/20 - 1/50. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).|
|Flow Cyt||Use 1-2µg for 106 cells.
(1/10 dilution on bone marrow and kidney tissue).
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration. PubMed: 20472681|
|ICC/IF||Use at an assay dependent concentration.|
FunctionXenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Tissue specificityHighly expressed in placenta. Low expression in small intestine, liver and colon.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.
modificationsGlycosylation-deficient ABCG2 is normally expressed and functional.
Cellular localizationCell membrane.
- Information by UniProt
- ABC transporter antibody
- ABC15 antibody
- ABCG 2 antibody
All lanes : Anti-BCRP/ABCG2 antibody [BXP-53] (ab24115) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
BCRP/ABCG2 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Overlay histogram showing HEK293 cells stained with ab24115 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24115, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
ab24115 staining BCRP/ABCG2 in murine mesenchymal stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.1% Triton X and then blocked using 1% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/25 for 15 hours at 25°C. The secondary antibody used was a donkey anti-rat IgG conjugated to an Alexa Fluor® used at a 1/100 dilution.
This product has been referenced in:
- Wang W et al. SOX2OT variant 7 contributes to the synergistic interaction between EGCG and Doxorubicin to kill osteosarcoma via autophagy and stemness inhibition. J Exp Clin Cancer Res 37:37 (2018). WB ; Human . Read more (PubMed: 29475441) »
- Zhang W et al. Rab23 promotes the cisplatin resistance of ovarian cancer via the Shh-Gli-ABCG2 signaling pathway. Oncol Lett 15:5155-5160 (2018). Read more (PubMed: 29552151) »