Recombinant
RabMAb

Recombinant Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Rabbit recombinant monoclonal BCRP/ABCG2 antibody [EPR20080]. Validated in WB, IHC, Flow Cyt and tested in Mouse, Rat, Human. Cited in 2 publication(s). Independently reviewed in 1 review(s).

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Overview

  • Product name

    Anti-BCRP/ABCG2 antibody [EPR20080]
    See all BCRP/ABCG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20080] to BCRP/ABCG2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human BCRP/ABCG2 aa 1-300. The exact sequence is proprietary.
    Database link: Q9UNQ0

  • Positive control

    • WB: HepG2 whole cell lysate, the lysate was prepared in 1% SDS Hot Lysate buffer and boiled before loading; A549 whole cell lysate, the lysate was prepared in RIPA lysate buffer and boiled before loading; Human placenta lysate. IHC-P: Human placenta, liver and glioma tissues; Mouse and rat liver tissues. Flow Cyt: A549 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).

Sample preparation may require optimisation depending on the tissue/cell lysates.

We don’t recommend this antibody for mouse and rat in WB. In our hands mouse and rat tissues showed additional bands.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/100.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

  • Tissue specificity

    Highly expressed in placenta. Low expression in small intestine, liver and colon.

  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.

  • Post-translational
    modifications

    Glycosylation-deficient ABCG2 is normally expressed and functional.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ABC transporter antibody
    • ABC15 antibody
    • ABCG 2 antibody
    • ABCG2 antibody
    • ABCG2_HUMAN antibody
    • ABCP antibody
    • ATP binding cassette sub family G (WHITE) member 2 antibody
    • ATP binding cassette transporter G2 antibody
    • ATP-binding cassette sub-family G member 2 antibody
    • BCRP antibody
    • BCRP1 antibody
    • BMDP antibody
    • Breast cancer resistance protein antibody
    • CD338 antibody
    • CDw338 antibody
    • CDw338 antigen antibody
    • EST157481 antibody
    • GOUT1 antibody
    • MGC102821 antibody
    • Mitoxantrone resistance associated protein antibody
    • Mitoxantrone resistance-associated protein antibody
    • MRX antibody
    • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
    • MXR antibody
    • MXR1 antibody
    • Placenta specific ATP binding cassette transporter antibody
    • Placenta specific MDR protein antibody
    • Placenta-specific ATP-binding cassette transporter antibody
    • UAQTL1 antibody
    see all

Images

  • Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line), whole cell lysate, the lysate was prepared in 1% SDS Hot Lysate buffer and boiled before loading
    Lane 2 : Huh7 (human liver hepatocellular carcinoma epithelial cell line), whole cell lysate, the lysate was prepared in 1% SDS Hot Lysate buffer and boiled before loading

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 72 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    Negative control: Huh7 (PMID: 26327240, PMID: 18820285).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on human placenta (PMID: 12237881). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate, the lysate was prepared in RIPA lysate buffer and boiled before loading
    Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate, the lysate was prepared in RIPA lysate buffer and was not boiled before loading

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 72 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

    Lane 1 : Human placenta, the lysate was prepared in RIPA lysate buffer and boiled before loading
    Lane 2 : Human placenta, the lysate was prepared in RIPA lysate buffer and was not boiled before loading

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 72 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of human liver (PMID: 12237881). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on capillaries of human glioma (PMID: 20216549). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of mouse liver (PMID: 12237881). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of rat liver (PMID: 12237881). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Flow Cytometry - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Flow cytometric analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) cell line labeling BCRP/ABCG2 with ab207732 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

References

This product has been referenced in:

  • Huang X  et al. Functional role of BTB and CNC Homology 1 gene in pancreatic cancer and its association with survival in patients treated with gemcitabine. Theranostics 8:3366-3379 (2018). Read more (PubMed: 29930735) »
  • Xu L  et al. Acridone suppresses the proliferation of human breast cancer cells in vitro via ATP-binding cassette subfamily G member 2. Oncol Lett 15:2651-2654 (2018). Read more (PubMed: 29434987) »
See all 3 Publications for this product

Publishing research using ab207732? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BCRP/ABCG2 antibody [EPR20080]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
Other
Specification
brain
Blocking step
BSA1% + DK serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Mrs. Francoise Geffroy

Verified customer

Submitted Jan 29 2018

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