Name: Anti-BCRP/ABCG2 antibody [EPR20080]
Brand: RabMAb
SKU: ab207732
Rating: 4 (5 reviews)

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Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20080] to BCRP/ABCG2
Suitable for: Flow Cyt (Intra), WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-BCRP/ABCG2 antibody [EPR20080]
See all BCRP/ABCG2 primary antibodies
Description

Rabbit monoclonal [EPR20080] to BCRP/ABCG2
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild-type A549, HepG2, THP-1 cell lysate; HepG2 whole cell lysate prepared in 1% SDS Hot Lysate buffer and boiled before loading; A549 whole cell lysate prepared in RIPA lysate buffer and not boiled before loading; Human placenta lysate. IHC-P: Human placenta, liver and glioma tissues; Mouse and rat liver tissues. Flow Cyt (intra): A549 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20080
Isotype

IgG
Research areas
- Neuroscience
- Development
- Neuroscience
- Processes

Alternative Versions
- Anti-BCRP/ABCG2 antibody [EPR20080] - BSA and Azide free (ab232517)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human ABCG2 knockout A549 cell line (ab259773)
KO cell lysates
- Human ABCG2 knockout A549 cell lysate (ab259778)
Recombinant Protein
- Recombinant Human BCRP/ABCG2 protein (ab152985)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(2)	1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). Sample preparation may require optimisation depending on the tissue/cell lysates. We don’t recommend this antibody for mouse and rat in WB. In our hands mouse and rat tissues showed additional bands.
IHC-P	(1)	1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
Flow Cyt (Intra) 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). Sample preparation may require optimisation depending on the tissue/cell lysates. We don’t recommend this antibody for mouse and rat in WB. In our hands mouse and rat tissues showed additional bands.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Tissue specificity

Highly expressed in placenta. Low expression in small intestine, liver and colon.
Sequence similarities

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.
Post-translational
modifications

Glycosylation-deficient ABCG2 is normally expressed and functional.
Cellular localization

Cell membrane.
Target information above from: UniProt accession Q9UNQ0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 9429 Human
- Entrez Gene: 26357 Mouse
- Entrez Gene: 312382 Rat
- Omim: 603756 Human
- SwissProt: Q9UNQ0 Human
- SwissProt: Q7TMS5 Mouse
- SwissProt: Q80W57 Rat
- Unigene: 480218 Human
- Unigene: 333096 Mouse
- Unigene: 13131 Rat
see all
Alternative names
- ABC transporter antibody
- ABC15 antibody
- ABCG 2 antibody
- ABCG2 antibody
- ABCG2_HUMAN antibody
- ABCP antibody
- ATP binding cassette sub family G (WHITE) member 2 antibody
- ATP binding cassette transporter G2 antibody
- ATP-binding cassette sub-family G member 2 antibody
- BCRP antibody
- BCRP1 antibody
- BMDP antibody
- Breast cancer resistance protein antibody
- CD338 antibody
- CDw338 antibody
- CDw338 antigen antibody
- EST157481 antibody
- GOUT1 antibody
- MGC102821 antibody
- Mitoxantrone resistance associated protein antibody
- Mitoxantrone resistance-associated protein antibody
- MRX antibody
- Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
- MXR antibody
- MXR1 antibody
- Placenta specific ATP binding cassette transporter antibody
- Placenta specific MDR protein antibody
- Placenta-specific ATP-binding cassette transporter antibody
- UAQTL1 antibody
see all

Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : ABCG2 knockout A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : THP-1 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 72 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab207732 observed at 70 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab207732 was shown to react with BCRP/ABCG2 in wild-type A549 cells in Western blot with loss of signal observed in ABCG2 knockout cell line ab259773 (ABCG2 knockout cell lysate ab259778). Wild-type A549 and ABCG2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab207732 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line), whole cell lysate, the lysate was prepared in 1% SDS Hot Lysate buffer and boiled before loading
Lane 2 : Huh7 (human liver hepatocellular carcinoma epithelial cell line), whole cell lysate, the lysate was prepared in 1% SDS Hot Lysate buffer and boiled before loading

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

Negative control: Huh7 (PMID: 26327240, PMID: 18820285).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on human placenta (PMID: 12237881). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate, the lysate was prepared in RIPA lysate buffer and boiled before loading
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate, the lysate was prepared in RIPA lysate buffer and was not boiled before loading

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

Lane 1 : Human placenta, the lysate was prepared in RIPA lysate buffer and boiled before loading
Lane 2 : Human placenta, the lysate was prepared in RIPA lysate buffer and was not boiled before loading

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of human liver (PMID: 12237881). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on capillaries of human glioma (PMID: 20216549). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of mouse liver (PMID: 12237881). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of rat liver (PMID: 12237881). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) cell line labeling BCRP/ABCG2 with ab207732 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab207732? Please let us know so that we can cite the reference in this datasheet.

ab207732 has been referenced in 18 publications.

Tang DH et al. Inosine induces acute hyperuricaemia in rhesus monkey (Macaca mulatta) as a potential disease animal model. Pharm Biol 59:175-182 (2021). PubMed: 33715593
Pan X et al. METTL3 promotes adriamycin resistance in MCF-7 breast cancer cells by accelerating pri-microRNA-221-3p maturation in a m6A-dependent manner. Exp Mol Med N/A:N/A (2021). PubMed: 33420414
Sui Y et al. Roles of NANOGP8 in cancer metastasis and cancer stem cell invasion during development of castration-resistant prostate cancer. Ann Transl Med 9:45 (2021). PubMed: 33553338
Wang C et al. Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance. Med Sci Monit 27:e927727 (2021). PubMed: 33524008
Sui Y et al. Insights into homeobox B9: a propeller for metastasis in dormant prostate cancer progenitor cells. Br J Cancer 125:1003-1015 (2021). PubMed: 34247196

View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Rat Tissue lysate - whole (Rat AR42J pancreatic cell line)

Gel Running Conditions

Reduced Denaturing

Loading amount

40 µg

Specification

Rat AR42J pancreatic cell line

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 05 2021

Application

Western blot

Sample

Human Cell lysate - whole cell (Placental and Fetal cell lines)

Gel Running Conditions

Reduced Denaturing (4-20%)

Loading amount

25 µg

Specification

Placental and Fetal cell lines

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Feb 01 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (18)

Customer reviews and Q&As

Western blot abreview for Anti-BCRP/ABCG2 antibody [EPR20080]

Western blot abreview for Anti-BCRP/ABCG2 antibody [EPR20080]

Post-translational
modifications