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    bcrpabcg2-antibody-epr20080-ab207732.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (5) Submit a question References (18)

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Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
  • Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
  • Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
  • Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
  • Flow Cytometry (Intracellular) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
  • Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20080] to BCRP/ABCG2
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Product image
Recombinant Human BCRP/ABCG2 protein (ab152985)
ELISA
Product image
Rabbit IgG ELISA Kit (ab187400)

View more associated products

Overview

  • Product name

    Anti-BCRP/ABCG2 antibody [EPR20080]
    See all BCRP/ABCG2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20080] to BCRP/ABCG2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type A549, HepG2, THP-1 cell lysate; HepG2 whole cell lysate prepared in 1% SDS Hot Lysate buffer and boiled before loading; A549 whole cell lysate prepared in RIPA lysate buffer and not boiled before loading; Human placenta lysate. IHC-P: Human placenta, liver and glioma tissues; Mouse and rat liver tissues. Flow Cyt (intra): A549 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20080
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Cancer
    • Drug resistance
    • MRP-related proteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-BCRP/ABCG2 antibody [EPR20080] - BSA and Azide free (ab232517)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ABCG2 knockout A549 cell line (ab259773)
  • KO cell lysates

    • Human ABCG2 knockout A549 cell lysate (ab259778)
  • Recombinant Protein

    • Recombinant Human BCRP/ABCG2 protein (ab152985)
  • Related Products

    • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100.

        ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (2)
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).

Sample preparation may require optimisation depending on the tissue/cell lysates.

We don’t recommend this antibody for mouse and rat in WB. In our hands mouse and rat tissues showed additional bands.

IHC-P (1)
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/100.

        ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).

Sample preparation may require optimisation depending on the tissue/cell lysates.

We don’t recommend this antibody for mouse and rat in WB. In our hands mouse and rat tissues showed additional bands.

IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
  • Tissue specificity

    Highly expressed in placenta. Low expression in small intestine, liver and colon.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • Post-translational
    modifications

    Glycosylation-deficient ABCG2 is normally expressed and functional.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q9UNQ0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9429 Human
    • Entrez Gene: 26357 Mouse
    • Entrez Gene: 312382 Rat
    • Omim: 603756 Human
    • SwissProt: Q9UNQ0 Human
    • SwissProt: Q7TMS5 Mouse
    • SwissProt: Q80W57 Rat
    • Unigene: 480218 Human
    • Unigene: 333096 Mouse
    • Unigene: 13131 Rat
    see all
  • Alternative names

    • ABC transporter antibody
    • ABC15 antibody
    • ABCG 2 antibody
    • ABCG2 antibody
    • ABCG2_HUMAN antibody
    • ABCP antibody
    • ATP binding cassette sub family G (WHITE) member 2 antibody
    • ATP binding cassette transporter G2 antibody
    • ATP-binding cassette sub-family G member 2 antibody
    • BCRP antibody
    • BCRP1 antibody
    • BMDP antibody
    • Breast cancer resistance protein antibody
    • CD338 antibody
    • CDw338 antibody
    • CDw338 antigen antibody
    • EST157481 antibody
    • GOUT1 antibody
    • MGC102821 antibody
    • Mitoxantrone resistance associated protein antibody
    • Mitoxantrone resistance-associated protein antibody
    • MRX antibody
    • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
    • MXR antibody
    • MXR1 antibody
    • Placenta specific ATP binding cassette transporter antibody
    • Placenta specific MDR protein antibody
    • Placenta-specific ATP-binding cassette transporter antibody
    • UAQTL1 antibody
    see all

Images

  • Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : ABCG2 knockout A549 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : THP-1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab207732 observed at 70 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab207732 was shown to react with BCRP/ABCG2 in wild-type A549 cells in Western blot with loss of signal observed in ABCG2 knockout cell line ab259773 (ABCG2 knockout cell lysate ab259778). Wild-type A549 and ABCG2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab207732 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line), whole cell lysate, the lysate was prepared in 1% SDS Hot Lysate buffer and boiled before loading
    Lane 2 : Huh7 (human liver hepatocellular carcinoma epithelial cell line), whole cell lysate, the lysate was prepared in 1% SDS Hot Lysate buffer and boiled before loading

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 72 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    Negative control: Huh7 (PMID: 26327240, PMID: 18820285).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on human placenta (PMID: 12237881). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate, the lysate was prepared in RIPA lysate buffer and boiled before loading
    Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate, the lysate was prepared in RIPA lysate buffer and was not boiled before loading

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 72 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    All lanes : Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution

    Lane 1 : Human placenta, the lysate was prepared in RIPA lysate buffer and boiled before loading
    Lane 2 : Human placenta, the lysate was prepared in RIPA lysate buffer and was not boiled before loading

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 72 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of human liver (PMID: 12237881). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on capillaries of human glioma (PMID: 20216549). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of mouse liver (PMID: 12237881). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of rat liver (PMID: 12237881). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Flow Cytometry (Intracellular) - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) cell line labeling BCRP/ABCG2 with ab207732 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)
    Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (18)

Publishing research using ab207732? Please let us know so that we can cite the reference in this datasheet.

ab207732 has been referenced in 18 publications.

  • Tang DH  et al. Inosine induces acute hyperuricaemia in rhesus monkey (Macaca mulatta) as a potential disease animal model. Pharm Biol 59:175-182 (2021). PubMed: 33715593
  • Pan X  et al. METTL3 promotes adriamycin resistance in MCF-7 breast cancer cells by accelerating pri-microRNA-221-3p maturation in a m6A-dependent manner. Exp Mol Med N/A:N/A (2021). PubMed: 33420414
  • Sui Y  et al. Roles of NANOGP8 in cancer metastasis and cancer stem cell invasion during development of castration-resistant prostate cancer. Ann Transl Med 9:45 (2021). PubMed: 33553338
  • Wang C  et al. Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance. Med Sci Monit 27:e927727 (2021). PubMed: 33524008
  • Sui Y  et al. Insights into homeobox B9: a propeller for metastasis in dormant prostate cancer progenitor cells. Br J Cancer 125:1003-1015 (2021). PubMed: 34247196
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-5 of 5 Abreviews

Western blot abreview for Anti-BCRP/ABCG2 antibody [EPR20080]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat AR42J pancreatic cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Rat AR42J pancreatic cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 05 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BCRP/ABCG2 antibody [EPR20080]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (PLACENTA)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH - 9
Permeabilization
No
Specification
PLACENTA
Blocking step
abcam Protein block ab156024 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ananth Kumar Kammala

Verified customer

Submitted Apr 26 2021

Western blot abreview for Anti-BCRP/ABCG2 antibody [EPR20080]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Placental and Fetal cell lines)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
25 µg
Specification
Placental and Fetal cell lines
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 01 2021

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BCRP/ABCG2 antibody [EPR20080]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (BRAIN)
Antigen retrieval step
Other
Permeabilization
Yes - triton 1%
Specification
BRAIN
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Francoise Geffroy

Verified customer

Submitted Jun 03 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BCRP/ABCG2 antibody [EPR20080]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
Other
Specification
brain
Blocking step
BSA1% + DK serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Francoise Geffroy

Verified customer

Submitted Jan 29 2018

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