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RecombinantRabMAb

Recombinant Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (11)

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Western blot - Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)
  • Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2099(2)] to BCRP/ABCG2
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-BCRP/ABCG2 antibody [EPR2099(2)]
    See all BCRP/ABCG2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2099(2)] to BCRP/ABCG2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, JAR, 293T, HepG2, and Human placenta lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2099(2)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Cancer
    • Drug resistance
    • MRP-related proteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-BCRP/ABCG2 antibody [EPR2099(2)] - BSA and Azide free (ab239926)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human BCRP/ABCG2 protein (ab152985)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
    • Tissue specificity

      Highly expressed in placenta. Low expression in small intestine, liver and colon.
    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.
    • Post-translational
      modifications

      Glycosylation-deficient ABCG2 is normally expressed and functional.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession Q9UNQ0 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9429 Human
      • Omim: 603756 Human
      • SwissProt: Q9UNQ0 Human
      • Unigene: 480218 Human
      • Alternative names

        • ABC transporter antibody
        • ABC15 antibody
        • ABCG 2 antibody
        • ABCG2 antibody
        • ABCG2_HUMAN antibody
        • ABCP antibody
        • ATP binding cassette sub family G (WHITE) member 2 antibody
        • ATP binding cassette transporter G2 antibody
        • ATP-binding cassette sub-family G member 2 antibody
        • BCRP antibody
        • BCRP1 antibody
        • BMDP antibody
        • Breast cancer resistance protein antibody
        • CD338 antibody
        • CDw338 antibody
        • CDw338 antigen antibody
        • EST157481 antibody
        • GOUT1 antibody
        • MGC102821 antibody
        • Mitoxantrone resistance associated protein antibody
        • Mitoxantrone resistance-associated protein antibody
        • MRX antibody
        • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
        • MXR antibody
        • MXR1 antibody
        • Placenta specific ATP binding cassette transporter antibody
        • Placenta specific MDR protein antibody
        • Placenta-specific ATP-binding cassette transporter antibody
        • UAQTL1 antibody
        see all

      Images

      • Western blot - Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)
        Western blot - Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)
        All lanes : Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : JAR lysate
        Lane 3 : 293T lysate
        Lane 4 : HepG2 lysate
        Lane 5 : Human placenta lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 72 kDa

      • Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)
        Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (11)

      Publishing research using ab108312? Please let us know so that we can cite the reference in this datasheet.

      ab108312 has been referenced in 11 publications.

      • Gomes I  et al. Expression of receptor activator of NFkB (RANK) drives stemness and resistance to therapy in ER+HER2- breast cancer. Oncotarget 11:1714-1728 (2020). PubMed: 32477461
      • Ding Y  et al. Levistolide A synergistically enhances doxorubicin-induced apoptosis of k562/dox cells by decreasing MDR1 expression through the ubiquitin pathway. Oncol Rep 41:1198-1208 (2019). PubMed: 30483812
      • Wang X  et al. Fatty acid receptor GPR120 promotes breast cancer chemoresistance by upregulating ABC transporters expression and fatty acid synthesis. EBioMedicine 40:251-262 (2019). PubMed: 30738829
      • Simbulan-Rosenthal CM  et al. CD133 Is Associated with Increased Melanoma Cell Survival after Multikinase Inhibition. J Oncol 2019:6486173 (2019). PubMed: 31379943
      • Nakano T  et al. Hepatitis C virus core protein triggers abnormal porphyrin metabolism in human hepatocellular carcinoma cells. PLoS One 13:e0198345 (2018). PubMed: 29856826
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-BCRP/ABCG2 antibody [EPR2099(2)]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (human liver)
      Permeabilization
      No
      Specification
      human liver
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Fixative
      methanol:acetone (1:1 ratio)
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 30 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-BCRP/ABCG2 antibody [EPR2099(2)]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (ES-derived hepatocytes)
      Permeabilization
      Yes - BSA diluted in PBS/0.1% Tween
      Specification
      ES-derived hepatocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Fixative
      Methanol
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 12 2013

      Question

      " rel="stylesheet" />
      Hello,

      I want to buy anti-BCRP(ab108312) and anti-MRP1(ab32574). Please tell me the positive controls for them, respecitively.
      I hope to hear from you as soon as possible. Thanks very much!

      Best wishes!

      Donghai Jiang


      2012-03-15
      jdh8499

      Read More

      Abcam community

      Verified customer

      Asked on Mar 15 2012

      Answer

      Thank you for contacting us.

      For ab108312, you could use human fetal liver (ab29890), JAR, 293T (ab95494), HepG2 (ab7900) or human placenta (ab29745) lysates as a positive control.

      For ab32574, you could consider using MRP-1 transfected HEK293 cell lysate as positive control.


      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Mar 15 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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