Recombinant
RabMAb

Recombinant Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)

Reviews (2)Q&A (1)References (10)

Overview

  • Product name

    Anti-BCRP/ABCG2 antibody [EPR2099(2)]
    See all BCRP/ABCG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2099(2)] to BCRP/ABCG2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human BCRP/ABCG2 aa 150-250 (intracellular). The exact sequence is proprietary.

  • Positive control

    • Human fetal liver, JAR, 293T, HepG2, and Human placenta lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab108312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

    • Tissue specificity

      Highly expressed in placenta. Low expression in small intestine, liver and colon.

    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.

    • Post-translational
      modifications

      Glycosylation-deficient ABCG2 is normally expressed and functional.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ABC transporter antibody
        • ABC15 antibody
        • ABCG 2 antibody
        • ABCG2 antibody
        • ABCG2_HUMAN antibody
        • ABCP antibody
        • ATP binding cassette sub family G (WHITE) member 2 antibody
        • ATP binding cassette transporter G2 antibody
        • ATP-binding cassette sub-family G member 2 antibody
        • BCRP antibody
        • BCRP1 antibody
        • BMDP antibody
        • Breast cancer resistance protein antibody
        • CD338 antibody
        • CDw338 antibody
        • CDw338 antigen antibody
        • EST157481 antibody
        • GOUT1 antibody
        • MGC102821 antibody
        • Mitoxantrone resistance associated protein antibody
        • Mitoxantrone resistance-associated protein antibody
        • MRX antibody
        • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
        • MXR antibody
        • MXR1 antibody
        • Placenta specific ATP binding cassette transporter antibody
        • Placenta specific MDR protein antibody
        • Placenta-specific ATP-binding cassette transporter antibody
        • UAQTL1 antibody
        see all

      Images

      • Western blot - Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)
        Western blot - Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)
        All lanes : Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : JAR lysate
        Lane 3 : 293T lysate
        Lane 4 : HepG2 lysate
        Lane 5 : Human placenta lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 72 kDa

      References

      This product has been referenced in:

      • Ding Y  et al. Levistolide A synergistically enhances doxorubicin-induced apoptosis of k562/dox cells by decreasing MDR1 expression through the ubiquitin pathway. Oncol Rep 41:1198-1208 (2019). Read more (PubMed: 30483812) »
      • Wang X  et al. Fatty acid receptor GPR120 promotes breast cancer chemoresistance by upregulating ABC transporters expression and fatty acid synthesis. EBioMedicine 40:251-262 (2019). Read more (PubMed: 30738829) »
      See all 10 Publications for this product

      Publishing research using ab108312? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-BCRP/ABCG2 antibody [EPR2099(2)]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (human liver)
      Permeabilization
      No
      Specification
      human liver
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Fixative
      methanol:acetone (1:1 ratio)
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 30 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-BCRP/ABCG2 antibody [EPR2099(2)]

       Poor
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (ES-derived hepatocytes)
      Permeabilization
      Yes - BSA diluted in PBS/0.1% Tween
      Specification
      ES-derived hepatocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Fixative
      Methanol
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 12 2013

      Question

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      Hello,

      I want to buy anti-BCRP(ab108312) and anti-MRP1(ab32574). Please tell me the positive controls for them, respecitively.
      I hope to hear from you as soon as possible. Thanks very much!

      Best wishes!

      Donghai Jiang


      2012-03-15
      jdh8499

      Read More
      Answer

      Thank you for contacting us.

      For ab108312, you could use human fetal liver (ab29890), JAR, 293T (ab95494), HepG2 (ab7900) or human placenta (ab29745) lysates as a positive control.

      For ab32574, you could consider using MRP-1 transfected HEK293 cell lysate as positive control.


      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

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