Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-BCRP/ABCG2 antibody [EPR2099(2)]
See all BCRP/ABCG2 primary antibodies -
DescriptionRabbit monoclonal [EPR2099(2)] to BCRP/ABCG2
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human BCRP/ABCG2 aa 150-250 (intracellular). The exact sequence is proprietary.
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Positive control
- Human fetal liver, JAR, 293T, HepG2, and Human placenta lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR2099(2)
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab108312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 72 kDa. |
Target
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FunctionXenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
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Tissue specificityHighly expressed in placenta. Low expression in small intestine, liver and colon.
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Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
Post-translational
modificationsGlycosylation-deficient ABCG2 is normally expressed and functional. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 9429 Human
- Omim: 603756 Human
- SwissProt: Q9UNQ0 Human
- Unigene: 480218 Human
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Alternative names
- ABC transporter antibody
- ABC15 antibody
- ABCG 2 antibody
see all
Images
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![Western blot - Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)](https://www.abcam.com/ps/products/108/ab108312/Images/BCRPABCG2-Primary-antibodies-ab108312-1.jpg)
Western blot - Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)All lanes : Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : JAR lysate
Lane 3 : 293T lysate
Lane 4 : HepG2 lysate
Lane 5 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa
Protocols
References
This product has been referenced in:
- Pan X et al. Downregulation of eIF4G by microRNA-503 enhances drug sensitivity of MCF-7/ADR cells through suppressing the expression of ABC transport proteins. Oncol Lett 13:4785-4793 (2017). WB . Read more (PubMed: 28599480) »
- Zhan Y et al. Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy. Oncol Lett 12:2511-2516 (2016). Read more (PubMed: 27698820) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"