Product nameAnti-BCS1L antibody
See all BCS1L primary antibodies
DescriptionRabbit polyclonal to BCS1L
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human BCS1L aa 33-240.
RKGVQLGLVAFRRHYMITLEVPARDRSYAWLLSWLTRHSTRTQHLSVETS YLQHESGRISTKFEFVPSPGNHFIWYRGKWIRVERSREMQMIDLQTGTPW ESVTFTALGTDRKVFFNILEEARELALQQEEGKTVMYTAVGSEWRPFGYP RRRRPLNSVVLQQGLADRIVRDVQEFIDNPKWYTDRGIPYRRGYLLYGPP GCGKSSFI
Database link: Q9Y276
- WB: Mouse kidney, liver and brain lysates; A549, HEK-293T and HeLa whole cell lysates. IHC-P: Human glioma cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- Mouse liver tissue lysate - total protein (ab29301)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (ab4022)
- A549 whole cell lysate (ab7910)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab230479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/20 - 1/200.|
RelevanceBCS1L (BCS1-like) is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. The protein does not contain a mitochondrial targeting sequence but experimental studies confirm that it is imported into mitochondria. Mutations in this gene are associated with mitochondrial complex III deficiency and the GRACILE syndrome. Two alternatively spliced transcripts encoding the same protein have been described.
Cellular localizationMitochondrial. Mitochondrion inner membrane. Single-pass membrane protein.
- BCS 1 antibody
- BCS 1L antibody
- BCS antibody
All lanes : Anti-BCS1L antibody (ab230479) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse brain lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48 kDa
Paraffin-embedded human glioma cancer tissue stained for BCS1L using ab230479 at 1/100 dilution in immunohistochemical analysis.
ab230479 has not yet been referenced specifically in any publications.