Key features and details
- Rabbit polyclonal to BCS1L
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-BCS1L antibody
See all BCS1L primary antibodies
DescriptionRabbit polyclonal to BCS1L
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human BCS1L aa 33-240.
RKGVQLGLVAFRRHYMITLEVPARDRSYAWLLSWLTRHSTRTQHLSVETS YLQHESGRISTKFEFVPSPGNHFIWYRGKWIRVERSREMQMIDLQTGTPW ESVTFTALGTDRKVFFNILEEARELALQQEEGKTVMYTAVGSEWRPFGYP RRRRPLNSVVLQQGLADRIVRDVQEFIDNPKWYTDRGIPYRRGYLLYGPP GCGKSSFI
Database link: Q9Y276
- WB: Mouse kidney, liver and brain lysates; A549, HEK-293T and HeLa whole cell lysates. IHC-P: Human glioma cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- Mouse liver tissue lysate - total protein (ab29301)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (ab4022)
- A549 whole cell lysate (ab7910)
Our Abpromise guarantee covers the use of ab230479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/20 - 1/200.|
RelevanceBCS1L (BCS1-like) is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. The protein does not contain a mitochondrial targeting sequence but experimental studies confirm that it is imported into mitochondria. Mutations in this gene are associated with mitochondrial complex III deficiency and the GRACILE syndrome. Two alternatively spliced transcripts encoding the same protein have been described.
Cellular localizationMitochondrial. Mitochondrion inner membrane. Single-pass membrane protein.
- BCS 1 antibody
- BCS 1L antibody
- BCS antibody
All lanes : Anti-BCS1L antibody (ab230479) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse brain lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48 kDa
Paraffin-embedded human glioma cancer tissue stained for BCS1L using ab230479 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230479 has not yet been referenced specifically in any publications.