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Please can you provide details of how the synthetic immunising peptide was made, with particular attention to the formation of the cysteine bridges prior to immunisation.
Asked on Oct 24 2005
Thank you for your enquiry. Further to correspondence with the source of this antiserum I have been informed that the antiserum was raised against tricycle human ß-Defensin 2 antigen. Unfortunately they could not provide details of whether the antisera had been tested against the native protein or your protein standards. I have therefore not been able to find evidence that these sera have been tested against the native form of this protein. I would definitely be interested to know how these sera performed against the denatured forms of your standards.
Answered on Oct 27 2005