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Recently, I purchased a BD-3 antibody (ab14422) PO#SL0911375AC and encountered a problem. While running my experiment I used an anti-mouse and an anti-rabbit antibody for detection of your BD-3 antibody. To my surprse, I found that the anti-rabbit antibody cross reacted with the mouse monoclonal antibody purchased from your company. Could you please verify/confirm that the mouse monoclonal BD-3 antibody mouse or rabbit. Please feel free to contact me if further information is required.
Asked on Sep 29 2011
Thank you for contacting us. The BD-3 antibody is a mouse monoclonal antibody. Is the anti-rabbit secondary also an Abcam antibody? If it has not been pre-adsorbed to remove reactivity with mouse IgG, it is possible it cross-reacts with mouse IgG, even though it is raised against rabbit IgG, since immunoglobulins are conserved to varying extent across many species.
Answered on Sep 29 2011