For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Thank you for your response. The anti-rabbit secondary antibody is not from abcam, but another comercialized antibody from another company. We have used the anti-rabbit antibody for other experiemtns as well and have not found it to cross react with other species of antibodies. Unfortunately, within this experiment the data showed over a 100% cross reactivity. I can provide you with more information if you require it.
Asked on Sep 29 2011
Thank you for the clarification. We are confident that ab14422 is a mouse monoclonal antibody. Have you used the secondary with other mouse antibodies, isotype IgG1? That is the isotype (IgG1) of this clone (L3-18b-E1). I recommend contacting the company that produces the secondary. They may have mouse IgG cross-reactivity data for the lot you have. If you need an anti-rabbit secondary that will not react with mouse IgG1, I will be happy to recommend one from our catalogue. We have several anti-rabbit antibodies that are pre-adsorbed to remove reactivity with mouse IgG and other species, with several different conjugations, for example ab96899: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96899).
Answered on Sep 29 2011