Anti-B2R antibody (ab236093)
Key features and details
- Rabbit polyclonal to B2R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-B2R antibody
See all B2R primary antibodies -
Description
Rabbit polyclonal to B2R -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human B2R aa 300 to the C-terminus.
Database link: P30411 -
Positive control
- WB: MCF7 whole cell lysate. IHC-P: Human brain tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/5000. Predicted molecular weight: 44 kDa.
|
|
IHC-P |
1/100 - 1/300.
|
Notes |
---|
WB
1/500 - 1/5000. Predicted molecular weight: 44 kDa. |
IHC-P
1/100 - 1/300. |
Target
-
Function
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Ubiquitous. Widespread in normal smooth muscle tissue and neurons. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Bradikinin receptor subfamily. BDKRB2 sub-subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 624 Human
- Omim: 113503 Human
- SwissProt: P30411 Human
- Unigene: 654542 Human
- Unigene: 726620 Human
-
Alternative names
- B2 antibody
- B2 bradykinin receptor antibody
- B2BKR antibody
see all
Images
-
Anti-B2R antibody (ab236093) at 1/500 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa -
Paraffin-embedded human brain tissue stained for B2R using ab236093 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab236093 has not yet been referenced specifically in any publications.