• Product name
    Anti-BDNF antibody [35928.11]
    See all BDNF primary antibodies
  • Description
    Mouse monoclonal [35928.11] to BDNF
  • Host species
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human BDNF (rhBDNF) expressed in Sf 21 insect cells.

  • Positive control
    • Brain tissue lysate
  • General notes
    Endotoxin: <10 ng/mg by LAL method.

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.



Our Abpromise guarantee covers the use of ab10505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 27.8 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.


  • Function
    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificity
    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in disease
    Bulimia nervosa 2
    Congenital central hypoventilation syndrome
  • Sequence similarities
    Belongs to the NGF-beta family.
  • Post-translational
    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Abrineurin antibody
    • ANON2 antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • BULN2 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all


This product has been referenced in:
  • Jasim H  et al. Saliva as a medium to detect and measure biomarkers related to pain. Sci Rep 8:3220 (2018). Read more (PubMed: 29459715) »
  • Deng FY  et al. SNP rs6265 regulates protein phosphorylation and osteoblast differentiation and influences BMD in humans. J Bone Miner Res 28:2498-507 (2013). Read more (PubMed: 23712400) »

See all 5 Publications for this product

Customer reviews and Q&As

Western blot
Human Tissue lysate - other (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (4-15% stain free gel)
Loading amount
20 µg
Skeletal muscle
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 6°C

Abcam user community

Verified customer

Submitted Feb 15 2016

Gracias por contactarnos de nuevo.

¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y gu...

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Thank you for contacting us.

I am sorry to hear you have been experiencing problems with Anti-BDNF antibody ab10505 in rat samples.

Unfortunately, this antibody has not been tested with rat samples and therefore we cannot guarante...

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Thank you very much for providing your protocol details. I think the 60kDa band you originally observed is a result of the homodimerization of BDNF in the samples in your first experiment so this is very promising (for example the datasheet of anoth...

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