Anti-BDNF antibody (ab222060)

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Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab222060)

    Key features and details

    • Rabbit polyclonal to BDNF
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-BDNF antibody
      See all BDNF primary antibodies

    • Description

      Rabbit polyclonal to BDNF

    • Host species

      Rabbit

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey

    • Immunogen

      Recombinant fragment corresponding to Human BDNF aa 154-223.
      Sequence:

      KTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRH WNSQCRTTQSYVRALTMDSK


      Database link: P23560
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • ICC/IF: U-2 OS cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab222060 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use a concentration of 0.25 - 2 µg/ml.

    Fixation/Permeabilization: PFA/Triton X-100.

    Target

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab222060)
      Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab222060)

      PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for BDNF (green) using ab222060 at 4 µg/ml in ICC/IF.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab222060? Please let us know so that we can cite the reference in this datasheet.

    ab222060 has not yet been referenced specifically in any publications.

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