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  1. Link

    bdnf-antibody-ab222060.pdf

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Neuroscience Neurology process Growth and Development Neurotrophins
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Anti-BDNF antibody (ab222060)

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Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab222060)

    Key features and details

    • Rabbit polyclonal to BDNF
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
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    Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794)

    View more associated products

    Overview

    • Product name

      Anti-BDNF antibody
      See all BDNF primary antibodies
    • Description

      Rabbit polyclonal to BDNF
    • Host species

      Rabbit
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey
    • Immunogen

      Recombinant fragment corresponding to Human BDNF aa 154-223.
      Sequence:

      KTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRH WNSQCRTTQSYVRALTMDSK


      Database link: P23560
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • ICC/IF: U-2 OS cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 40% Glycerol (glycerin, glycerine), PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Growth and Development
      • Neurotrophins
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Huntington's disease
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other
      • Cardiovascular
      • Atherosclerosis
      • Diabetes associated
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Other
      • Metabolism
      • Types of disease
      • Diabetes
      • Metabolism
      • Types of disease
      • Obesity

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794)
    • Related Products

      • Recombinant human BDNF protein (Animal Free) (ab217473)

    Applications

    Our Abpromise guarantee covers the use of ab222060 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use a concentration of 0.25 - 2 µg/ml.

    Fixation/Permeabilization: PFA/Triton X-100.

    Target

    • Function

      During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • Tissue specificity

      Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • Involvement in disease

      Bulimia nervosa 2
      Congenital central hypoventilation syndrome
    • Sequence similarities

      Belongs to the NGF-beta family.
    • Post-translational
      modifications

      The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P23560 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 493690 Cat
      • Entrez Gene: 503511 Chimpanzee
      • Entrez Gene: 617701 Cow
      • Entrez Gene: 403461 Dog
      • Entrez Gene: 100009689 Horse
      • Entrez Gene: 627 Human
      • Entrez Gene: 12064 Mouse
      • Entrez Gene: 397495 Pig
      • Entrez Gene: 24225 Rat
      • Omim: 113505 Human
      • SwissProt: Q9TST3 Cat
      • SwissProt: Q5IS78 Chimpanzee
      • SwissProt: Q95106 Cow
      • SwissProt: Q7YRB4 Dog
      • SwissProt: Q0EAB7 Horse
      • SwissProt: P23560 Human
      • SwissProt: P21237 Mouse
      • SwissProt: P14082 Pig
      • SwissProt: P23363 Rat
      • SwissProt: P14082 Rhesus monkey
      • SwissProt: Q06225 Rhesus monkey
      • Unigene: 502182 Human
      • Unigene: 1442 Mouse
      • Unigene: 11266 Rat
      see all
    • Alternative names

      • Abrineurin antibody
      • ANON2 antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • BULN2 antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab222060)
      Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab222060)

      PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for BDNF (green) using ab222060 at 4 µg/ml in ICC/IF.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab222060? Please let us know so that we can cite the reference in this datasheet.

    ab222060 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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