Overview

  • Product name

  • Description

    Rabbit polyclonal to BDNF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human BDNF (N terminal).
    Database link: P23560

  • Positive control

    • WB: HeLa and HEK-293T cell lysates; Mouse brain lysate. ICC/IF: SK-N-BE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 27.9 kDa (predicted molecular weight: 27.9 kDa).

Target

  • Function

    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificity

    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in disease

    Bulimia nervosa 2
    Congenital central hypoventilation syndrome
  • Sequence similarities

    Belongs to the NGF-beta family.
  • Post-translational
    modifications

    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Abrineurin antibody
    • ANON2 antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • BULN2 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for BDNF (green) using ab223354 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min RT, 5 min RT. 

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223354 (D) Merge.

  • Anti-BDNF antibody (ab223354) at 1/1000 dilution + Mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG HRP at 1/2000 dilution

    Predicted band size: 27.9 kDa
    Observed band size: 27.9 kDa


    Exposure time: 12 minutes


    Blocking buffer: 5% milk in TBST.

    Color Development: TMB solution.

    Primary and secondary antibodies were incubated for 1 hour at RT.

  • All lanes : Anti-BDNF antibody (ab223354) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG HRP at 1/2000 dilution

    Predicted band size: 27.9 kDa
    Observed band size: 27.9 kDa


    Exposure time: 12 minutes


    Blocking buffer: 5% milk in TBST.

    Color Development: TMB solution.

    Primary and secondary antibodies were incubated for 1 hour at RT.

References

ab223354 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223354.
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