Overview

  • Product name

  • Description

    Rabbit polyclonal to BDNF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Cat, Dog, Human, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide within Human BDNF (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated synthetic peptide.
    Database link: P23560

  • Positive control

    • IHC-P: Adult mouse retina tissue, Rat brain tissue. WB: Mouse and rat brain tissue extract,Transfected (+) 293T whole cell extracts ICC/IF: DIV9 rat E18 primary cortical neuron cells.
  • General notes

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

Properties

Applications

Our Abpromise guarantee covers the use of ab226843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Dilution is assay dependent.

IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Detects a band of approximately 28 kDa.

Target

  • Function

    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificity

    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in disease

    Bulimia nervosa 2
    Congenital central hypoventilation syndrome
  • Sequence similarities

    Belongs to the NGF-beta family.
  • Post-translational
    modifications

    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Abrineurin antibody
    • ANON2 antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • BULN2 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

Images

  • All lanes : Anti-BDNF antibody (ab226843) at 1/500 dilution (4? overnight)

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Additional bands at: 17 kDa (possible mature (processed) protein)



    12% SDS-PAGE. 

    Running: 80V, 15min; 140V, 40 min. Transfer: Semi-dry, 18 V, 60 min (NC embrane). Blocking: 5% non-fat milk in TBST, RT, 60 minutes. Washing:  5 ml TBST, 4 x 5 min. ECL exposure.    

     

  • Immunocytochemistry/ Immunofluorescence analysis of DIV9 rat E18 primary cortical neuron cells labeling BDNF with ab226843 at 1:500 (green). Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Cells were counterstained with DAPI (blue) and a Tau antibody diluted at 1/500 (yellow). 

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling BDNF with ab226843 at 1/500 dilution at 4°C overnight. Antigen Retrieval:Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30min. Blocking:1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 minutes. Secondary antibody incubation: ABC HRP Kit (Rabbit IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB. 

  • Immunohistochemical analysis of paraffin-embedded adult mouse retina tissue labeling BDNF with ab226843 at 1/250 dilution (green). Counter nuclear stained with DAPI (blue) and a beta III Tubulin antibody diluted at 1/500 (red). 

  • All lanes : Anti-BDNF antibody (ab226843) at 1/1000 dilution

    Lane 1 : Non-transfected (–) 293T whole cell extracts
    Lane 2 : Transfected (+) 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    15% SDS-PAGE

References

This product has been referenced in:

  • Zhang DD  et al. Vildagliptin, a DPP4 inhibitor, alleviates diabetes-associated cognitive deficits by decreasing the levels of apoptosis-related proteins in the rat hippocampus. Exp Ther Med 15:5100-5106 (2018). Read more (PubMed: 29805536) »
See 1 Publication for this product

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