Key features and details
- Mouse monoclonal [BS375] to BDNF
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
FormLyophilized:Spin vial briefly before opening. Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives. After reconstitution divide into aliquots and store at -20ºC for a higher stability. Antibody contains no preservatives. Store at 4ºC with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Constituents: PBS, 3% Trehalose
Lyophilized without preservatives.
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab228505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µl for 106 cells.|
FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
Involvement in diseaseBulimia nervosa 2
Congenital central hypoventilation syndrome
Sequence similaritiesBelongs to the NGF-beta family.
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG).
- Information by UniProt
- Abrineurin antibody
- ANON2 antibody
- BDNF antibody
Flow cytometric analysis of methanol-fixed (10 minutes on ice), 0.1% Tween-20-permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling BDNF with ab228505 at 2 µg per 106 cells (black) compared with a Rabbit IgG Control (white). Goat anti-Mouse PE at 1/100 dilution was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228505 has not yet been referenced specifically in any publications.