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RecombinantRabMAb

Recombinant Anti-BDNF antibody [EPR1292] (ab108319)

  • Datasheet
  • SDS
Reviews (8)Q&A (2)References (204)

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Western blot - Anti-BDNF antibody [EPR1292] (ab108319)
  • Western blot - Anti-BDNF antibody [EPR1292] (ab108319)
  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)
  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319)
  • Flow Cytometry - Anti-BDNF antibody [EPR1292] (ab108319)
  • Western blot - Anti-BDNF antibody [EPR1292] (ab108319)
  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)
  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319)
  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319)
  • Anti-BDNF antibody [EPR1292] (ab108319)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1292] to BDNF
  • Suitable for: Flow Cyt, WB, IHC-P, IHC-Fr, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-BDNF antibody [EPR1292]
    See all BDNF primary antibodies
  • Description

    Rabbit monoclonal [EPR1292] to BDNF
  • Host species

    Rabbit
  • Specificity

    This product may cross react with the following family members: NGF beta, neurotrophin 3, neurotrophin 4. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab182199)

  • Positive control

    • WB: Human, rat and mouse brain, hippocampus and cerebellum lysates; IHC-P: Human brain tissue, human bladder cancer tissue; ICC/IF: HeLa cells; Flow Cyt: HeLa cells; IHC-Fr: Mouse and Rat cerebrum tissue, Hu cerebral cortex.
  • General notes

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1292
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Neurotrophins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Signal Transduction
    • Antibodies
    • bdnf

Associated products

  • Alternative Versions

    • Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
    • Alexa Fluor® 488 Anti-BDNF antibody [EPR1292] (ab222113)
    • Alexa Fluor® 647 Anti-BDNF antibody [EPR1292] (ab222321)
    • Alexa Fluor® 568 Anti-BDNF antibody [EPR1292] (ab222322)
    • Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunizing Peptide (Blocking)

    • Human BDNF peptide (ab182199)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/30.
WB (5)
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.Can be blocked with Human BDNF peptide (ab182199).
IHC-P (1)
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

For unpurified use at 1/100 - 1/250 dilution. 

IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

ICC/IF (1)
1/500.

For unpurified use at 1/750 dilution. 

Notes
Flow Cyt
1/30.
WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.Can be blocked with Human BDNF peptide (ab182199).
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

For unpurified use at 1/100 - 1/250 dilution. 

IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

ICC/IF
1/500.

For unpurified use at 1/750 dilution. 

Target

  • Function

    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificity

    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in disease

    Bulimia nervosa 2
    Congenital central hypoventilation syndrome
  • Sequence similarities

    Belongs to the NGF-beta family.
  • Post-translational
    modifications

    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P23560 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 627 Human
    • Entrez Gene: 12064 Mouse
    • Entrez Gene: 24225 Rat
    • Omim: 113505 Human
    • SwissProt: P23560 Human
    • SwissProt: P21237 Mouse
    • SwissProt: P23363 Rat
    • Unigene: 502182 Human
    • Unigene: 1442 Mouse
    • Unigene: 11266 Rat
    see all
  • Alternative names

    • Abrineurin antibody
    • ANON2 antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • BULN2 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

Images

  • Western blot - Anti-BDNF antibody [EPR1292] (ab108319)
    Western blot - Anti-BDNF antibody [EPR1292] (ab108319)
    All lanes : Anti-BDNF antibody [EPR1292] (ab108319) at 1/1000 dilution (unpurified)

    Lane 1 : Human hippocampus lysate
    Lane 2 : Rat hippocampus lysate
    Lane 3 : Mouse hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Gt anti Rb IR680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Additional bands at: 15 kDa (possible mature (processed) protein), 28 kDa (possible multimer), 35 kDa, 45 kDa (possible immature (unprocessed)). We are unsure as to the identity of these extra bands.



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with unpurified ab108319 (1/1000) overnight at 4°C. Ab8245 (mouse anti-GAPDH; 0.05 ug/mL) was included as a loading control. Antibody binding was detected using goat anti-rabbit IgG IR-680 (green) and goat anti-mouse IgG IR800 (red) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

  • Western blot - Anti-BDNF antibody [EPR1292] (ab108319)
    Western blot - Anti-BDNF antibody [EPR1292] (ab108319)
    All lanes : Anti-BDNF antibody [EPR1292] (ab108319) at 1/1000 dilution (Purified)

    Lane 1 : Human brain lysates with 5% NFDM/TBST
    Lane 2 : Mouse brain lysates with 5% NFDM/TBST
    Lane 3 : Rat brain lysates with 5% NFDM/TBST
    Lane 4 : Human hippocampus lysates with 5% NFDM/TBST
    Lane 5 : Mouse hippocampus lysates with 5% NFDM/TBST
    Lane 6 : Rat hippocampus lysates with 5% NFDM/TBST
    Lane 7 : Human cerebellum lysates with 5% NFDM/TBST
    Lane 8 : Mouse cerebellum lysates with 5% NFDM/TBST
    Lane 9 : Rat cerebellum lysates with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15-45 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)
    Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)

    IHC image of BDNF staining in a section of frozen normal human cerebral cortex performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab108319, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)
    Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)
    Immunohistochemistry (Frozen sections) analysis of rat cerebral cortex tissue sections labeling BDNF with Purified ab108319 at 1/100 (2.8 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling BDNF with Purified ab108319 at 1:500 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Flow Cytometry - Anti-BDNF antibody [EPR1292] (ab108319)
    Flow Cytometry - Anti-BDNF antibody [EPR1292] (ab108319)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with purified ab108319 at 1:30 dilution (10 µg/ml) (red). Cells were fixed with 80% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-BDNF antibody [EPR1292] (ab108319)
    Western blot - Anti-BDNF antibody [EPR1292] (ab108319)
    Different batches of ab108319 were tested on Mouse brain lysate at 0.3 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 14-45 kDa.
  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)
    Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)
    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling BDNF with Purified ab108319 at 1/100 (2.8 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319)
    Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with Purified ab108319 at 1:500 (0.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319)

    Immunohistochemical analysis of paraffin-embedded human brain tissue using unpurified ab108319 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319)
    Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319)This image is courtesy of an Abreview submitted by Kirk McManus (1894904)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling BDNF with unpurified ab108319 at a dilution of 1/750. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.

    The antibody produces a strong, golgi-associated labelling pattern in both PF and MeOH fixed samples.

    See Abreview

  • Anti-BDNF antibody [EPR1292] (ab108319)
    Anti-BDNF antibody [EPR1292] (ab108319)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (204)

Publishing research using ab108319? Please let us know so that we can cite the reference in this datasheet.

ab108319 has been referenced in 204 publications.

  • Ye J  et al. Tau inhibits PKA by nuclear proteasome-dependent PKAR2a elevation with suppressed CREB/GluA1 phosphorylation. Aging Cell 19:e13055 (2020). PubMed: 31668016
  • Ye J  et al. MicroRNA-496 suppresses tumor cell proliferation by targeting BDNF in osteosarcoma. Exp Ther Med 19:1425-1431 (2020). PubMed: 32010318
  • Liu D  et al. Protective Effects of Chlorogenic Acid on Cerebral Ischemia/Reperfusion Injury Rats by Regulating Oxidative Stress-Related Nrf2 Pathway. Drug Des Devel Ther 14:51-60 (2020). PubMed: 32021091
  • Cheng Y  et al. RasGRF1 participates in the protective effect of tanshinone IIA on depressive like behaviors of a chronic unpredictable mild stress induced mouse model. Gene 754:144817 (2020). PubMed: 32473965
  • Wang F  et al. miR-210 enhances mesenchymal stem cell-modulated neural precursor cell migration. Mol Med Rep 21:2405-2414 (2020). PubMed: 32323777
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

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1-8 of 8 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BDNF antibody [EPR1292]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Permeabilization
No
Specification
cerebellum
Fixative
formalin
Read More

Msr. Emmanuel Bouchaert

Verified customer

Submitted Oct 06 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-BDNF antibody [EPR1292]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Read More

Dr. Kirk Mcmanus

Verified customer

Submitted Sep 29 2016

Western blot abreview for Anti-BDNF antibody [EPR1292]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SHSY5Y cells)
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycin gel)
Loading amount
40 µg
Specification
SHSY5Y cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jan 12 2021

Western blot abreview for Anti-BDNF antibody [EPR1292]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Balb/C mice brain)
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycin gel)
Loading amount
40 µg
Specification
Balb/C mice brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jan 12 2021

Western blot abreview for Anti-BDNF antibody [EPR1292]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (16%)
Loading amount
25 µg
Specification
Hippocampus
Blocking step
LiCor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 05 2020

Western blot abreview for Anti-BDNF antibody [EPR1292]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (BRAIN)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
10 µg
Treatment
clioquinol
Specification
BRAIN
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Dr. Michele D'angelo

Verified customer

Submitted Feb 07 2020

Western blot abreview for Anti-BDNF antibody [EPR1292]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Endometrium (eutopic and ectopic))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
Endometrium (eutopic and ectopic)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted May 31 2017

Immunohistochemistry free floating abreview for Anti-BDNF antibody [EPR1292]

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain tissue, immersion fixed in 4% PFA, cut at 50)
Specification
brain tissue, immersion fixed in 4% PFA, cut at 50
Read More

Dr. Tom Smulders

Verified customer

Submitted Oct 11 2018

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