Application
Western blot
Sample
Mouse Tissue lysate - whole (BRAIN)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
10 µg
Treatment
clioquinol
Specification
BRAIN
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: MILK 5% in TBS-T 0.1%
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL pico
Exposure
40 second(s)
Bands
Specific: 30 kDa
Additional data
PubMed ID
29950603: View
PROF. Michele D'angelo
Verified customer
Submitted Feb 07 2020