Recombinant
RabMAb

Recombinant Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

Overview

  • Product name

    Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free
    See all BDNF primary antibodies
  • Description

    Rabbit monoclonal [EPR1292] to BDNF - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BDNF. The exact sequence is proprietary.
    (Peptide available as ab182199)

  • Positive control

    • WB: U87-MG, C6, SH-SY5Y, Neuron-2a, PC-12 cell lysate, brain tissue lysate. IHC-P: Human brain tissue. ICC/IF: HeLa cells
  • General notes

    ab206035 is the Low endotoxin, azide-free version of ab108319 This format is designed for in vitro and in vivo studies, including neutralization, blocking or activation/proliferation.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.Can be blocked with Human BDNF peptide (ab182199).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • Tissue specificity

      Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • Involvement in disease

      Bulimia nervosa 2
      Congenital central hypoventilation syndrome
    • Sequence similarities

      Belongs to the NGF-beta family.
    • Post-translational
      modifications

      The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Abrineurin antibody
      • ANON2 antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • BULN2 antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    Images

    • Co-immunofluorescence staining against tyrosine hydroxylase (TH; green), nuclear Dapi (blue) and BDNF (red, ab108319) in coronal sections from fetal (SNc) and post-natal (VTA) WT and Rgs6−/− mice. Scale bar 20 µm.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

    • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with purified ab108319 at 1:30 dilution (10 µg/ml) (red). Cells were fixed with 80% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with Purified ab108319 at 1:500 (0.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling BDNF with Purified ab108319 at 1:500 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling BDNF with unpurified ab108319 at a dilution of 1/750. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.

      The antibody produces a strong, golgi-associated labelling pattern in both PF and MeOH fixed samples.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

    • This IHC data was generated using the same anti-BDNF antibody clone, EPR1292, in a different buffer formulation (cat# ab108319).

      Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108319 at 1/100

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • This ICC/IF data was generated using the same anti-BDNF antibody clone, EPR1292, in a different buffer formulation (cat# ab108319).

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling BDNF with ab108319 at a dilution of 1/750. Cells were fixed with Paraformaldehyde and permeabilised with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.

      The antibody produces a strong, golgi-associated labelling pattern in both PF and MeOH fixed samples.

    References

    This product has been referenced in:

    • Yu YC  et al. Resveratrol Improves Brain-Gut Axis by Regulation of 5-HT-Dependent Signaling in the Rat Model of Irritable Bowel Syndrome. Front Cell Neurosci 13:30 (2019). Read more (PubMed: 30800058) »
    • Feng Y  et al. Bone marrow stromal cells promote neuromotor functional recovery, via upregulation of neurotrophic factors and synapse proteins following traumatic brain injury in rats. Mol Med Rep 16:654-660 (2017). WB ; Rat . Read more (PubMed: 28560414) »
    See all 2 Publications for this product

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