Product nameAnti-BDP / ARID3B antibody
See all BDP / ARID3B primary antibodies
DescriptionRabbit polyclonal to BDP / ARID3B
SpecificityBlock in 3% milk
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
- F9 Mouse Embryonic Carcinoma, E14tG2a (Mouse embryonic stem cell line), PC12 (Rat adrenal pheochromocytoma cell line)Whole Cell Lysate; Mouse Thymus, Testis, Thyroid Gland and Cerebellum Tissue Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab32481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionTranscription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A.
Tissue specificityExpressed in placenta, testis and leukocytes. Expressed in neuroblastoma. Present in K562 erythrocytic leukemia cells (at protein level).
Sequence similaritiesContains 1 ARID domain.
Contains 1 REKLES domain.
- Information by UniProt
- ARI3B_HUMAN antibody
- ARID domain containing protein 3B antibody
- ARID domain-containing protein 3B antibody
ICC/IF image of ab32481 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32481, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-BDP / ARID3B antibody (ab32481) at 1 µg/ml
Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : Thyroid Gland (Mouse) Tissue Lysate
Lane 3 : Testis (Mouse) Tissue Lysate
Lane 4 : Thymus (Mouse) Tissue Lysate
Lane 5 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 6 : Cerebellum (Mouse) Tissue Lysate
Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 61,70 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
Blocked in 3% milk.
IHC image of ab32481 staining in mouse testes formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32481, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.